Swetha Balakrishnan

6 Questions 6 Answers 0 Followers

Questions related from Swetha Balakrishnan

I am working on ISSR primers. I get bands for some samples and a smear in others. Why?

Hi, I am currently working on ISSR primers for my algal samples. When I did PCR for my samples along with some marine algal specimens, I could observe bands for the marine specimens and a smear...

05 May 2018 2,684 3 View

Which is a very successful procedure for extraction of genomic DNA from herbarium sample?

Greeting to all researchers! Can anyone suggest me with a successful procedure for extraction of genomic DNA from herbarium material of Red algae (Rhodophyceae). So far we are successful in...

03 March 2017 9,215 6 View

Is there any expected range of GC content variation for identificattion of sub species?

I have done rbcL sequencing of two populations of the same species of algae. On calculating the GC content of the sequenced product I got a 1% difference between the two populations. Does this...

02 February 2017 1,773 3 View

Does chloroform melt ependorff or other larger centrifugr tubes?

Hi all! While doing my DNA isolation I encountered a new problem. After the addition of chloroform and during centrifugation, the ependorff and 50mL centrifuge tubes melted. Can anyone provide me...

01 January 2017 1,540 13 View

Does 16s rRNA sequencing of soil bacterium using two different sets of universal primers give different results?

I have a small doubt in sequencing. I had sequenced 16s rRna region of my bacterium in two labs. Both of them had used different sets of primers for the same sample and same region and inferred...

06 June 2016 7,349 6 View

Is it necessary to use spin coloum after following conventional method for DNA extraction?

I have extracted genomic DNA by conventional CTAB method. I need to do partial sequencing , for which, I was asked to purify my DNA using spin column. I have checked the purity of my DNA using...

04 April 2016 8,382 3 View