Is there any existing method, protocol or kit for gamma-glutamylcysteine synthetase assay in animal tissue?

Eliza Dec @Eliza-Dec

09 July 2015 1 10K Report

I know about ELISA kit but we are more interested in kinetic or absorbance measurement.

Sandip Chakraborty

Gamma Glutamylcysteine Synthetase ELISA Kits of Biocompare is available at present.

Apart from this, you can please refer to the following link:-

http://www.ncbi.nlm.nih.gov/pubmed/12044560

Badges
Science method

More Eliza Dec's questions See All

How to change the version of the article full-text pdf file?

How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...

30 July 2024 3,229 2 View

Help: Troubleshooting Metash UV-Vis (MetaSpec Pro), Negative Absorbances <230 nm?

Tl;dr - As of last week, the UV-Vis instrument at my job only records negative absorbance below 230 nm for any sample when zeroing methanol to correct for solvent. Attached spectra of the same...

03 April 2024 5,271 5 View

I'm writing a scientific report for the first time for uni. Which statistical test should I do and how?

The data consists of a control and the measurements taken at 2 weeks, then another control and measurements taken at 4 weeks. The data is for 2 groups so there's 2 sets of data for the different...

02 April 2023 3,385 2 View

How to interpret the Vickers hardness data?

I have done Vicker's hardness analysis with titanium and thin-film coated titanium and I'm trying to show the hardness of the coated titanium. I plotted the graph of hardness versus load from 0.1...

09 February 2022 1,146 10 View

Could someone explain the calculation for osteogenic differentiation media?

Dexamethasone is 100nM Glycerophosphate is 10mM Ascorbic acid is 50ug/ml. But I'm not understanding how to calculate the working concentration and mixing with media to prepare osteogenic media.

08 March 2021 8,442 4 View

Growing condition for Streptococcus intermedius?

I'm going to work on anaerobic bacteria for the first time. For this organism, THB agar is used. But I have less knowledge on the protocol for growing such bacteria using a CO2 incubator. Can...

16 February 2021 2,727 6 View

How to calculate the degradation rate in percentage ?

I performed the BCA assay to calculate protein content over the time period. However, I'm not understanding how I can calculate the degradation rate in percentage at each time point? What formula...

10 January 2021 9,827 3 View

How to prevent precipitation of keratin and chitosan composite?

When 2% chitosan dissolved in 2% acetic acid is mixed with keratin solution of pH 9, then little precipitation is apparent. How to get rid of such precipitate? Or it doesn't affect while forming a...

08 September 2020 4,172 3 View

Calvarial rat model for osseointegration?

I'm trying to use modified titanium disc to study osseointegration in animal study, however, I just got confused with calvarial rat model as this model has been studied mostly for bone...

15 May 2020 5,268 0 View

Why peaks appear down in case of absorbance measurement in FTIR?

I tested the Titanium disc in FTIR after treating it with chemicals. But the absorbance peaks are showing down. I don't know what is wrong going on.

07 November 2019 2,244 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View