Journal of Research in Humanities and Social Science. Is a real magazine?

Dear colleagues: I have received many emails from a magazine called ournal of Research in Humanities and Social Science. I have gone to the website of this journal, and I read "some" indexes but,...

13 December 2020 6,208 6 View

How can I connect a thermocouple to an oscilloscope?

I would like to measure temperature with a very high acquisition rate during a test that takes place in under 1ms. I was thinking of using a thermocouple and an oscilloscope. Which kind of signal...

26 October 2020 1,736 6 View

How efficient are HiTrap desalting columns for separation of proteins from free fluorescent labels?

I am looking for a reliable and fast method for separation of protein-fluorescent probe conjugates from the respective free fluorophores present in labeling reactions. HiTrap desalting columns...

06 September 2020 8,039 8 View

PhyloCode now a mediatic debate?

The recent official publication of PhyloCode has caught my attention, not for new arguments being presented for a system that has already been extensively discussed and, as I see it, whose...

04 July 2020 726 10 View

How to write a password automatically in a bash script?

I have a script that download multiples files from a server, but for each one it requires me to write a password. These are 1000, so it would be laborious to write 1000 times the password. How...

02 April 2020 852 2 View

How to define parametric modulators for multiple conditions in SPM?

Dear RG Community, I am performing an SPM analysis in which I've got 8 conditions plus the baseline in an event-related experiment. Each condition has 4 trials, totalling 32 trials per run. There...

02 April 2020 7,060 1 View

Alkali Resistance of SS316, Nickel and Hastelloy?

Does anyone know or may anyone assist me to find which of these materials (Stainless Steel 316, Nickel, or Hastelloy) is the most resistant to a highly concentrated NaOH solution (>27 mol/L)...

26 March 2020 6,875 6 View

Recommendations/tips on publishing datasets?

In my research about vibration of hermetic compressors, I'm creating a dataset that I intend to make it available once I publish the paper it refers to. Do you have any tips or recommendations on...

18 March 2020 7,803 5 View

Tools for ncRNA in bacteria?

Hello, I am trying to determine if a region present in a Streptomyces genome acts as a ncRNA that affects gene expression. Which are the best tools to analyze sequences for this? Thanks!

24 November 2019 5,270 3 View

In Limma package, why should I linearize my data in some analyses?

tl;dr: Why data linearization is applied in the identification o differentially expressed genes/proteins? I am new to data analysis of big data like proteomics. As far as I know, a simple t-test...

16 November 2019 8,441 4 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

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22 February 2021 9,932 11 View