How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to count the number of figure when reusing figure??

Dear all I'm preparing a review paper and confused about terms for reusing figure in other papers... Can you please help me for 2 question..?? (1) As i know, the cost of reusing figure are...

28 December 2020 1,515 3 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

Can I autoclave d-mannose?

Hello everyone, I was wondering whether I should autoclave or filter d-mannose solution. In our laboratory, we autoclave glucose separately when we make YPD media for yeast culture. So, I am...

08 October 2020 7,068 1 View

Is there any general way to make polymer hydrophobic?

Now I try to make hydrophobic pillar array using Resin 3D printing (Formlab's 'Surgical Guide Resin'). But actually I don't know exactly what the resin is made of. But as we know it is the mixture...

05 July 2020 3,860 5 View

Does topology from ATB support FF other than Gromos?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

07 June 2020 8,989 2 View

How can I use a molecule from ATB in Gromacs?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

06 June 2020 2,112 4 View

I want measure intracellular pH change in sperm with BCECF-AM. It requires pH standard?

This is my first time to try measure intracellular pH but it is confusing about protocol. I have BCECF/AM (216254, CAS 117464-70-7, Sigma) and i understood protocol briefly as below. 1. Load...

04 June 2020 6,392 6 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

Does anyone know about analysis of 3D mapping and depth profile for wooden object?

I have 1cm cubic size wood samples. inside of wood, there are S and S-organic, inorganic compounds randomly. I'd like to figure out the quantities of S distribution non-destructively. First, I...

20 February 2020 9,303 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

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23 February 2021 4,014 3 View

Passivation of chromium cast Iron in high temperature?

14 February 2021 2,157 3 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

Is there any books or journal that specifically discussed TPR in detail?

I am designing a metal oxide catalyst and among many characterization of this sample, TPR emerges quite significantly. The brief explanation in journal is limited to the sample that is...

21 January 2021 6,346 1 View

How cane capture of Cl, K , Na, in the process of biomass gasification, I want to let these elements enter the solid slag phase but not enter the gas?

I want to fixation Cl, K, Na in gasification slag, which type of catalyst can be used?

13 January 2021 2,145 2 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

How to calculate the concentration for as prepared nanomaterials?

if we are taking 2 materials of different concentrations (one is the precursor and the other one is the stabilizing agent) for synthesizing nanoparticles as a catalyst, how can I calculate the...

08 January 2021 516 4 View

How to choose proper spindle for measurement of thixotropic behavior of ceramic slurry?

Hello, we are studying on thixotropic behavior of ceramic slurry . We will perform the thixotropic behavior analysis of ceramic slurry with the density of 1,7 g/ml by using BROOKFIELD DV2T. How to...

08 January 2021 1,776 5 View