Is there any database available for pathogens or probiotic bacterial species ?

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

How to resolve Gaussian error termination " Error termination via Lnk1e in F:\G16W\l103.exe" for TS(QST3)?

When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...

21 February 2021 3,198 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How can I calculate volume fraction of MWCNT from a nano hybrid composite ?

I am adding 0.1wt percent MWCNT to glass fiber epoxy composite and want to determine the volume fraction of the MWCNT from this composite but I don't know how to calculate it. can anyone help me...

09 February 2021 8,883 3 View

Functionality on MWCNT can possible without using vacuum oven?

I want to synthesis functional MWCNT from pristine MWCNT using acid treatment but I haven't vacuum oven for drying but I have a normal hot air oven. So may it possible to use hot air oven for...

02 February 2021 8,475 3 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How can we measure disease severity of a pathogen in a crop?

severity index, measuring method and procedure detailed

01 February 2021 1,160 11 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

Does use of hand sanitizer negatively impact the skin microbiome?

Hello, Most hand sanitizers contain 60-80% alcohol. Alcohol destroys organisms including bacteria, fungi, protists, and enveloped viruses by breaking down the plasma membrane. In recent...

16 January 2021 6,923 11 View

Can someone help me identify the bands?

The samples are replicates (4) of total RNA from HEK-293 T cells. The ladder is a 1kBp ladder on a 1% native agarose gel buffered with 1x TAE. From my searches, there should be two sharp bands at...

15 January 2021 4,712 4 View

WURSS-11 - how to apply in research practice?

Hello, in the framework of my master's thesis, I would like to assess the efficacy of probiotic on upper respiratory tract infection (URTI) incidence and symptom ratings in athletes under...

07 January 2021 2,284 1 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

How do mRNA vaccines work and what differentiates the technology from the rest?

Most vaccines contain an infectious pathogen or a part of it, but mRNA vaccines deliver the genetic instructions for our cells to make viral or bacterial proteins themselves. Our immune system...

25 December 2020 3,982 4 View

Covid (or any) vaccines: differential effects on transmission/pathology?

Hi folks, can anyone provide me with explanations or references regarding why a vaccine would block disease symptoms, but not onward transmission of the pathogen? Leaving aside variations in...

22 December 2020 7,613 4 View

Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View