Hi, I am looking for cystine measurement in mouse serum and urine. But, I could not find suitable ELISA kit for this measurement. As cystine can be converted into cysteine by the breakdown of disulphide bond (thiol group) and few Bio-companies have a cysteine ELISA assay kit to measure mouse serum and urine. The assay principle is based on the cleavage of thiol group in reduced cysteine to produce that emits a stable signal.
Does anyone have idea about cystine/cysteine measurement in mouse? Can we measure cysteine and correlate with cystine?
The most accurate and preferred method of detection of these amino acids (Cystine, dibasic amino acids) is HPLC, however, we do not have this facility and looking for alternatives.
Regards,
Nirmal
Jay Lee Can we measure mouse serum/urine cysteine and compare results with cystine. I am wondering how cysteine and cystine levels in serum and urine effects the formation of cystinuria (cystine stones). Thanks
If you have a reliable way to measure Cysteine, you can use this assay to determine both Cysteine and Cystine by measuring your sample with and without prior reduction with a reducing agent that does not interfere with your assay, e.g. TCEP.
Annemarie Honegger Thanks. Do you have any links and details about TCEP.
TCEP is a reducing agent that does not depend on sulfhydryl groups
https://en.wikipedia.org/wiki/TCEP
https://www.sigmaaldrich.com/catalog/product/aldrich/c4706?lang=de®ion=CH. It should be compatible for an ELISA based assay, as long as you do not use it in concentrations large enough to mess up your antibody. It is not compatible with cysteine assays based on disulfide exchange, such as Ellman's reagent Article Quantification of Thiols and Disulfides
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