Hi,
Is there any alternative method to HPLC to measure cystine concentration in mouse urine? I have found HPLC is mostly used to measure cystine in human urine.
In case of mice there are limited studies regarding cystine measurement.
Thank you.
Unless you lack an HPLC at your disposal, the method for cystiene measurements from human urine should be transferable to mouse urine as well. There will obviously be some amount of standardization that will be necessary, but it should not be too difficult.
Divya Ramesh Could you please elaborate on cystine measurement in urine. Do you know any kits or analyser test available? Many thanks.
Nirmal Prasad Bhatt, Just a quick google search shows that you can buy amino acid derivatization and analysis kits from companies like Waters (for HPLC). https://www.waters.com/webassets/cms/library/docs/3acqtag.pdf
If you do not want to buy the AQC derivatizing agent (it is expensive), you could prepare it in-house from its constituents.
If you do not want to use HPLC, another short search brings up a whole whole of cysteine analysis kits.
https://www.google.co.in/search?client=opera&hs=At8&ei=c0I1Xa6VNsf6z7sPv-ub0Ao&q=cysteine+kit&oq=cysteine+kit&gs_l=psy-ab.3..0i22i30l5.112156.115728..115981...0.0..0.156.609.0j5......0....1..gws-wiz.......0i67j0i7i30j0j0i7i10i30j0i10j0i20i263.NRhKZ5w4o4w&ved=0ahUKEwjumvPp3sfjAhVH_XMBHb_1BqoQ4dUDCAo&uact=5
I hope this helps.