Is there any commercially available porcine gelatin ELISA kit?

04 April 2016 1 3K Report

If there is no such kit, what protocol/ELISA method can be used to detect and quantify porcine gelatin by using gelatin antibody

Cássio Prinholato da Silva

http://www.antibodyresource.com/search/ElisaKits/ef194e88-43e4-543b-86d0-17f0eabc6674/gelatin

Badges
Science method

More Muhammad Rizwan's questions See All

Is it possible to graft amine or methacrylate groups to Graphene Oxide?

For amine conjugation: Would EDC chemistry work given that the GO has carboxylic groups? For methacrylation: Is it possible to methacrylate the OH groups of GO? How easy or difficult are the above...

10 November 2014 3,319 5 View

How do you do localized (in the center only) cell seeding on your samples?

I have 1cmx1cm TCPS samples and I only want to seed cells in the center (~0.3cm2 area). I have tried making PDMS rings and gluing it to the samples but there is always a leakage issue. If I do not...

08 September 2013 4,200 6 View

How do you purify hydrogels for cell seeding?

I am preparing gelatin hydrogel and need to purify it after its methacrylation. I'll use that as a substrate for cell seeding so I want to remove any unreacted components from the gelatin...

31 December 2012 9,512 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View