Hello, does anybody know if there is any commercially available hyperlipemic serum for cell culture? It would be rather easier to obtain (and presumably more standardised) than serum from WHHL rabbits or rats/rabbits on high fat diet. I would like to check the effect of such serum on the MSC in vitro differentiation. There are lipid supplements available (eg. from Sigma) but they do not represent the whole repertoire of lipids and lipoproteins found in the sera of atherosclerotic patients.
You can probably get lipid fraction of the serum for free at any peritoneal dialysis clinic for dyslipidemia. It's a waste for them and I am sure there are some clinics in London, but I am not sure what are the legal issues in UK. Just a thought.
I know that the Harefield Hospital trust near Uxbridge has a centre for LDL apheresis of familial hypercholesterolemia patients - not sure if you can recover the LDL after the procedure or if this would be suitable for your experiments...
- Badges
- Science topic