You could reference the paper below.

https://repositorio.uc.cl/bitstream/handle/11534/13315/A%20real-time%20PCR-based%20strategy%20for%20the%20detection%20of%20Paenibacillus%20larvae%20vegetative%20cells%20and%20spores%20to%20improve%20the%20diagnosis%20and%20the%20screening%20of%20American%20foulbrood.pdf?sequence=1

In this paper, they use soil DNA kit.

Thanks for the reference! I will try with the kit.

I guess you can also try this KAPA express DNA extraction kit from KAPABIOSYSTEMS.

Hi Anna

Some useful references on attachment 1

https://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02840.x/full

2

https://www.genesig.com/assets/files/p_larvae.pdf

3

https://www.agrojournal.org/20/04-26.pdf

4

https://link.springer.com/content/pdf/10.1134/S0003683813010171.pdf

5

https://www.ncbi.nlm.nih.gov/pubmed/19559547

6

https://www.apidologie.org/articles/apido/full_html/2010/04/m09049/m09049.html

7

https://vetdergikafkas.org/pdf.php?&id=798

8

https://www.academia.edu/24252900/A_PCR_method_of_detecting_American_Foulbrood_Paenibacillus_larvae_in_winter_beehive_wax_debris

9

https://link.springer.com/content/pdf/10.1007/s13592-013-0225-6.pdf

10

https://vetdergikafkas.org/pdf.php?&id=798

11

http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.02.02_AMERICAN_FOULBROOD.pdf

12

https://www.tandfonline.com/doi/pdf/10.1080/13102818.2014.906826

13

http://www.microbiologyresearch.org/docserver/fulltext/micro/157/5/1474_mic044321.pdf?expires=1502168382&id=id&accname=guest&checksum=72505CD651AAC773BDCBF0C42C688DB8

14

http://www.conicet.gov.ar/new_scp/detalle.php?keywords=&id=30715&congresos=yes&detalles=yes&congr_id=2182340

15

http://www.ingenetix.com/en/vetmed-products/real-time-pcr-detection-bacteria-animal-pathogens/bactoreal-american-foulbrood-new

16

https://jhr.pensoft.net/article/7061/download/pdf/

17

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090914

18

http://www.globaledico.com/wp-content/uploads/2016/01/Artigo%20Vale%20do%20Ribeira%20ing.docx

19

https://is.cuni.cz/webapps/zzp/download/140022530/?lang=en