Hi,
I'm trying to extract DNA from P. larvae spores for PCR detection.
I know that there are some attempts (Bakonyi et al. 2003/ Alessandro et al. 2007), but I wonder if there is any comercially available kit to extract DNA from spores.
You could reference the paper below.
https://repositorio.uc.cl/bitstream/handle/11534/13315/A%20real-time%20PCR-based%20strategy%20for%20the%20detection%20of%20Paenibacillus%20larvae%20vegetative%20cells%20and%20spores%20to%20improve%20the%20diagnosis%20and%20the%20screening%20of%20American%20foulbrood.pdf?sequence=1
In this paper, they use soil DNA kit.
Thanks for the reference! I will try with the kit.
I guess you can also try this KAPA express DNA extraction kit from KAPABIOSYSTEMS.
Hi Anna
Some useful references on attachment 1
https://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02840.x/full
2
https://www.genesig.com/assets/files/p_larvae.pdf
3
https://www.agrojournal.org/20/04-26.pdf
4
https://link.springer.com/content/pdf/10.1134/S0003683813010171.pdf
5
https://www.ncbi.nlm.nih.gov/pubmed/19559547
6
https://www.apidologie.org/articles/apido/full_html/2010/04/m09049/m09049.html
7
https://vetdergikafkas.org/pdf.php?&id=798
8
https://www.academia.edu/24252900/A_PCR_method_of_detecting_American_Foulbrood_Paenibacillus_larvae_in_winter_beehive_wax_debris
9
https://link.springer.com/content/pdf/10.1007/s13592-013-0225-6.pdf
10
11
http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.02.02_AMERICAN_FOULBROOD.pdf
12
https://www.tandfonline.com/doi/pdf/10.1080/13102818.2014.906826
13
http://www.microbiologyresearch.org/docserver/fulltext/micro/157/5/1474_mic044321.pdf?expires=1502168382&id=id&accname=guest&checksum=72505CD651AAC773BDCBF0C42C688DB8
14
http://www.conicet.gov.ar/new_scp/detalle.php?keywords=&id=30715&congresos=yes&detalles=yes&congr_id=2182340
15
http://www.ingenetix.com/en/vetmed-products/real-time-pcr-detection-bacteria-animal-pathogens/bactoreal-american-foulbrood-new
16
https://jhr.pensoft.net/article/7061/download/pdf/
17
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090914
18
http://www.globaledico.com/wp-content/uploads/2016/01/Artigo%20Vale%20do%20Ribeira%20ing.docx
19
https://is.cuni.cz/webapps/zzp/download/140022530/?lang=en
Dear Forum, My master thesis investigates the disposition effect—where traders sell winning shares too early and hold onto losing shares too long—on social trading platforms (STPs) like eToro,...
08 July 2024 187 0 View
Could you explain to me, how do assay cascades work during the DMTA (Design-Make-Test-Analyze) cycle of Drug Discovery? What is involed in it? How many assays are usually present?
24 June 2024 7,245 4 View
So I am doing my thesis on the relation between cyberbullying perpetration and life satisfaction and if physical activity is a moderator. But in my moderation analysis the interaction is...
17 June 2024 6,400 2 View
Dear ResearchGate, I seem to have 2 independent profiles on your site which I do not know how could happen - can you, please, merge those into one? Both are on my name, but affiliations, however,...
04 June 2024 5,682 0 View
I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).
21 May 2024 4,964 4 View
Hello, I am currently trying to optimise a bacterial expression system using BL2 1DE3 bacteria and IPTG. In order to just have a quick look, whether I can see any protein overexpression on a...
04 May 2024 3,549 2 View
criminal procedure
29 April 2024 1,705 2 View
I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...
28 April 2024 2,113 3 View
Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...
18 April 2024 3,219 0 View
Good morning, I need to isolate dendrites from axons in my microchamber, I have already tried a protocol of Netrin and it failed. I would like to put Sema3a in my dendritic compartment in order...
26 March 2024 2,632 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View