Is there any comercially available kit to perform DNA extraction from Paenibacillus larvae spores?

More Anna Leidinger's questions See All

Is it Ok to decline to write a chapter for a IntechOpen to the lack of experience?

I have been asked by IntechOpen to write the chapter for a book they are going to publish, but as I don't have enough experience in the area they proposed me to write I declined their offer. Now...

24 February 2021 2,802 4 View

How to inerpret whether PCR went OK?

Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...

14 February 2021 3,129 8 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

EMOJI-POLL-3 (Emoji-Association): Can you join the survey?

https://docs.google.com/forms/d/e/1FAIpQLSfaaCWZZqMIhoz5yj_5dxggvb6-cISx3GUHssYUWZWKbpx3UA/viewform Dear colleagues! We ask you to participate in a series of surveys related to the scientific...

26 January 2021 5,969 3 View

The same lC-MS for Forensic Toxicological and Chemical expertises?

Dear Colleagues! How correct and appropriate is it to carry out a forensic toxicological examination of drugs and psychotropic substances in biological objects (in blood, urine, hair) and a...

24 January 2021 4,489 2 View

GC-Headspace (HS-GC) chromatography for Forensic toxicological and Food expertises?

Dear Colleagues! How correct and appropriate is it to conduct forensic toxicological and food examination of volatile substances (ethanol, methanol, propanol, ethyl glycol, etc.) in blood and...

24 January 2021 5,645 2 View

How to choose mouse cell line to work on?

Hey, I am looking to start working on mouse cell line for some genomics experiments. I have the following requirements: 1) Normal chromosomes composition. 2) Possible to transfect. 3) Possible to...

03 January 2021 4,387 2 View

How to describe the method described below?

I do research on the relationships between universities and local governments by asking the research question (how) can universities contribute to the design (and implementation) of public...

29 December 2020 4,522 12 View

How can I change the media for THP-1 cells without dislodging them from the surface of my well plate?

I am working with THP-1 cells at the moment and am having trouble with maintaining a high confluence once I seed them on a 24-well plate. I usually try to seed around 100,000 cells per well and...

29 December 2020 1,567 3 View

What glass vials do you use for small volumes (400ul-2000ul) that fit in the centrifuge?

I am planning some NMR experiments that require glass vials (due to chloroform). I am looking to find glass vials that would take 400-2000ul volumes, fit in the centrifuge and allow me to separate...

01 December 2020 3,259 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Hyeonhee Hong

You could reference the paper below.

https://repositorio.uc.cl/bitstream/handle/11534/13315/A%20real-time%20PCR-based%20strategy%20for%20the%20detection%20of%20Paenibacillus%20larvae%20vegetative%20cells%20and%20spores%20to%20improve%20the%20diagnosis%20and%20the%20screening%20of%20American%20foulbrood.pdf?sequence=1

In this paper, they use soil DNA kit.

Anna Leidinger

Thanks for the reference! I will try with the kit.

Kai Wong

I guess you can also try this KAPA express DNA extraction kit from KAPABIOSYSTEMS.

Laith Al-Ani

Hi Anna

Some useful references on attachment 1

https://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02840.x/full

https://www.genesig.com/assets/files/p_larvae.pdf

https://www.agrojournal.org/20/04-26.pdf

https://link.springer.com/content/pdf/10.1134/S0003683813010171.pdf

https://www.ncbi.nlm.nih.gov/pubmed/19559547

https://www.apidologie.org/articles/apido/full_html/2010/04/m09049/m09049.html

https://vetdergikafkas.org/pdf.php?&id=798

https://www.academia.edu/24252900/A_PCR_method_of_detecting_American_Foulbrood_Paenibacillus_larvae_in_winter_beehive_wax_debris

https://link.springer.com/content/pdf/10.1007/s13592-013-0225-6.pdf

http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.02.02_AMERICAN_FOULBROOD.pdf

https://www.tandfonline.com/doi/pdf/10.1080/13102818.2014.906826

http://www.microbiologyresearch.org/docserver/fulltext/micro/157/5/1474_mic044321.pdf?expires=1502168382&id=id&accname=guest&checksum=72505CD651AAC773BDCBF0C42C688DB8

http://www.conicet.gov.ar/new_scp/detalle.php?keywords=&id=30715&congresos=yes&detalles=yes&congr_id=2182340

http://www.ingenetix.com/en/vetmed-products/real-time-pcr-detection-bacteria-animal-pathogens/bactoreal-american-foulbrood-new

https://jhr.pensoft.net/article/7061/download/pdf/

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090914

http://www.globaledico.com/wp-content/uploads/2016/01/Artigo%20Vale%20do%20Ribeira%20ing.docx

https://is.cuni.cz/webapps/zzp/download/140022530/?lang=en