I should analyze polymerase activity of thermophilic DNA polymerase. Earlier I used for this purpose DE81 paper to collect DNA with incorporated radiolabeled nucleotide. However, now I haven't any more DE81 and need to replace it with some other equipment. I know, that CF/C filters can be used for DNA precipitation, but in that case trichloroacetic acid (TCA) should be used. Is there any analog of DE81 paper or possibility to avoid using TCA for polymerase activity assay?
Thanks. Igor.