10 October 2019 2 6K Report

Hello,

I am trying to determine solubility of a well known molecule in multiple solubilizers. Is there an openware where I can upload the structures of both (drug substance and solvent) and get a solubility prediction?

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Why do I get an amplification curve in negative samples without template? Can anyone list methods to detect contamination of kit or RNA or reagents?

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Should I consider a spliced version of the gene to build the qPCR primers or an unspliced version which has an intron?

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How can FPKM and RPKM be compared?

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His tagged protein not binding to Ni-NTA coulmn.

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Should I refold my his-tagged protein after Triton-X100 digestion?

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