How do you find odorant binding protein in a genome?

I am working on Black fly. I just received the genome fasta file. I want to find the odorant binding protein in the genome. Which software should I use? And who should I consider as my reference...

06 July 2014 7,546 9 View

How does one decide which nuclear hormone receptors are relevant for human pathogenicity? What genomic resources should I use?

In brugia malayi there are many NHR encoded in the genome. How does one pick the relevant one that cause host pathogenicity?

06 July 2014 9,376 1 View

How does one determine therapeutic potential of a nuclear hormone receptor?

When you are investigating a particular NHR signaling pathway for the worm development, how do you know whether it has therapeutic potential or not? Is the treatment likely toxic or not? Can you...

06 July 2014 5,145 1 View

How to identify operons using transcriptomic data?

Nematodes have unique genomic features for eukaryotes, such as the existence of operons. These features require special analytical methods in genomics. Suppose you are analyzing a transcriptome...

06 July 2014 2,651 5 View

Why do I get an amplification curve in negative samples without template? Can anyone list methods to detect contamination of kit or RNA or reagents?

I am using RNA extracted from whole adult female Brugia malayi worms for qPCR studies. I have been using iTaq Universal SYBR green RT kit for my qPCR. From the first run I am getting a separate...

04 May 2014 9,858 4 View

Should I consider a spliced version of the gene to build the qPCR primers or an unspliced version which has an intron?

I ran an RNAseq with Brugia malayi worms treated with a drug and untreated. I found a certain number of genes differentially expressed in the treated worms as compared to the untreated. I selected...

03 April 2014 764 7 View

How can FPKM and RPKM be compared?

I have two samples, one is drug-treated and the other is not. I ran MiSeq on my samples and generated FPKM values. Some other author had done similar studies earlier and I want to compare my FPKM...

02 March 2014 4,457 3 View

His tagged protein not binding to Ni-NTA coulmn.

I am expressing a 6xHis tagged protein at C-terminal. I can see it in the supernatent,washes and flowthrough . But it does not stick to the nickel column. I use PBS+10%glycerol as my buffer. I...

07 August 2013 3,685 22 View

Should I refold my his-tagged protein after Triton-X100 digestion?

I am working on ligand binding domains and I am expressing the protein in the yeast system. I have solubilized my protein using Triton-X100. I do know that it is a non-ionic detergent and it...

07 August 2013 8,359 17 View

Can anyone suggest a Ammonium sulphate cut protocol?

My protein is 43kDa. I want to use ammonium sulphate cut to precipate my protein. Can anyone suggest at what concentration saturation will I achieve precipitation of my protein? Further down the...

07 August 2013 9,494 10 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View