23 Questions 23 Answers 0 Followers
Questions related from Amruta Mhashilkar
Hello, I am trying to determine solubility of a well known molecule in multiple solubilizers. Is there an openware where I can upload the structures of both (drug substance and solvent) and get a...
10 October 2019 5,793 2 View
I am working on Black fly. I just received the genome fasta file. I want to find the odorant binding protein in the genome. Which software should I use? And who should I consider as my reference...
07 July 2014 7,609 9 View
When you are investigating a particular NHR signaling pathway for the worm development, how do you know whether it has therapeutic potential or not? Is the treatment likely toxic or not? Can you...
07 July 2014 5,221 1 View
Nematodes have unique genomic features for eukaryotes, such as the existence of operons. These features require special analytical methods in genomics. Suppose you are analyzing a transcriptome...
07 July 2014 2,739 5 View
In brugia malayi there are many NHR encoded in the genome. How does one pick the relevant one that cause host pathogenicity?
07 July 2014 9,440 1 View
I am using RNA extracted from whole adult female Brugia malayi worms for qPCR studies. I have been using iTaq Universal SYBR green RT kit for my qPCR. From the first run I am getting a separate...
05 May 2014 9,950 4 View
I ran an RNAseq with Brugia malayi worms treated with a drug and untreated. I found a certain number of genes differentially expressed in the treated worms as compared to the untreated. I selected...
04 April 2014 831 7 View
I have two samples, one is drug-treated and the other is not. I ran MiSeq on my samples and generated FPKM values. Some other author had done similar studies earlier and I want to compare my FPKM...
03 March 2014 4,566 3 View
I am expressing a 6xHis tagged protein at C-terminal. I can see it in the supernatent,washes and flowthrough . But it does not stick to the nickel column. I use PBS+10%glycerol as my buffer. I...
08 August 2013 3,893 22 View
My protein is 43kDa. I want to use ammonium sulphate cut to precipate my protein. Can anyone suggest at what concentration saturation will I achieve precipitation of my protein? Further down the...
08 August 2013 9,592 10 View
I am working on ligand binding domains and I am expressing the protein in the yeast system. I have solubilized my protein using Triton-X100. I do know that it is a non-ionic detergent and it...
08 August 2013 8,418 17 View
I want to determine ideal pH of my buffers to isolate my his tagged protein. The isoelectric point of my protein is 7.87. What must be the pH of my buffers to make the purification go smoothly.
07 July 2013 1,220 9 View
I have a Brugia protein. It is getting expressed in the pellet. I need to solubilize it in a way that it does not lose its activity and its should not get denatured in the process. I cannot even...
07 July 2013 8,665 1 View
I have to use Ni-NTA biosensors for my histagged protein. Has anyone used these biosensors before? Also, will having detergent (Triton-X 100) in the final protein elution have any deleterious on...
07 July 2013 2,346 1 View
I am purifying a his-tagged protein. My vector has the his tag and a V5 epitope. I am using anti-V5 antibody for my western. On the western blot, I see a band at the correct molecular weight, but...
06 June 2013 709 15 View
I have to find a 13 nucleotide sequence in a genome. I have the fasta file of the genome. But, I don't have the software that can open the file. Can anyone suggest to me software to open and...
06 June 2013 1,699 7 View
I am using wormbase to retrieve files for my project. It has an annotations.gff2 file. I need to open it in a software that can show me the scafolds and contigs in it. Can anyone please suggest to...
06 June 2013 3,090 5 View
I have cloned my insert using Gateway cloning into a his tagged vectors. Then I transformed BL-21 cells once I confirmed my insert. I grew cells with correct selectable marker. I induced the cells...
06 June 2013 7,398 9 View
I have a palindromic sequence that I need to find in Brugia malayi genome. It is only 13 nucleotides. I tried using BLASTn but it failed the character requirement. I need to find it upstream of...
05 May 2013 8,548 4 View
I need to identify certain sequences and look out for a pattern in my cDNA. Can anyone suggest me a software that can detect 3'UTRs etc?
05 May 2013 3,964 4 View
I am a doctoral student starting my work in transcriptomes. My lab has never done it and we are venturing in the new era of transcriptome studies. I need help understanding what I am doing and how...
05 May 2013 5,621 6 View
My cell line has endogenous expression of Mol.A and B. But, when I overexpress A, I want to know whether it is altering endogenous expression of B or not. What experiment should I conduct to...
04 April 2013 9,968 5 View
I have a cell line that expresses an endogenous protein. Now, if I transfect overexpression in the same protein, how do I know that the results I am getting are due to the overexpression protein?
04 April 2013 6,704 8 View
