After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Why do irreversible inhibitors only affect Vmax and not Km?

So I've been wondering why irreversible inhibitors only affect maximal velocity and not Km? Is there a...

19 January 2021 2,509 1 View

In the Venusian atmosphere why doesn't Nitrogen which is much lighter Carbon Dioxide float to the upper regions?

Carbon dioxide on Earth weighs 44 g/mol and has a density of around 1.98 kg/m3, about 1.53 times that of air. Nitrogen gas weighs 28 g/mol and has a density of 1.2506 g/L. During limnic eruptions...

15 January 2021 8,733 4 View

What type of cellular structures are these likely to be?

I have immunohistochemistry slides of human subcutaneous fat biopsy, with skin attached. Most of the structures are self-evident - skin, adipocytes, microvessels in transverse section (revealed by...

03 December 2020 4,238 7 View

Mata-analysis question: How to deal with multiple outcome scales used within individual studies?

Dear all, thanks for the help in advance. I am performing a met-analysis. Most of the studies included report the same outcome using multiple scales e.g. MMSE or CDRS. What is the best way of...

30 October 2020 8,629 5 View

Glucose dehydrogenase interference with glucokinase assay?

Hello all, I'm currently working with undergraduate student-researchers (there are no graduate students at my university) to purify and characterize the drug targetability of glucokinase from pig...

25 October 2020 3,553 7 View

What is the minimum number of timepoints for markov models?

For those who frequently work in markov models, I am interested in knowing the minimum number of time points/observations to get stable estimates of parameters. I have some follow ups, too:...

24 September 2020 9,659 3 View

How best to address statistical differences when there is no difference in the independent variable?

If an experiment includes, say, two groups and Condition 1 is identical between groups but Condition 2 differs between groups, what is the best course of action if one finds a significant...

17 September 2020 9,731 7 View

Can you re-use ethidium bromide post-stain solution more than once, or does ethidium bromide need to be added fresh each time?

When post-staining agarose gels in an ethidium bromide / water bath for 10 minutes, my labmate says she can re-use the post-stain solution 2-3 times before she has to add more ethidium bromide (at...

04 September 2020 680 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View