How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

I have genotype gvcf of 30GB , which is causing an issue in analysis. I was wondering if there is a way to get around the size issue ?

I have a genotype gvcf of significant size, which is causing an issue in analysis. I was wondering if there is a way to get around the size issue and run the genotype gvcf in R or do I need to...

09 February 2021 4,178 4 View

How stiffness matrix modification after hinge formation is done for pushover analysis?

Can anyone please explain this? How do I account for plastic hinges in my model and how do I modify the stiffness after plastic hinge is formed? Need a small example. Thank You

15 November 2020 1,967 3 View

How to analyze nominal/ordinal data in cases where you are testing multiple drugs/ infection against common risk factors?

chi-square or Fisher exact test I have a statistical question about chi-square and/or Fisher exact test. I have a cohort data studying the cause of infection in BRD with variables such as age,...

28 October 2020 2,589 1 View

What vehicle to be used for the drug loaded nano chitosan-pectin nanoencapsulated powder?

I want to know about vehicle or solution that I can use for the dosing of drug loaded nanochitosan-pectin nanoencapsulated powder?

25 October 2020 3,890 3 View

How to use 5 by 5 or 7 by 7 kernel size for a deep learning network with 3 by 3 kernels?

I am using a U-Net architecture with Xception net as encoder . The visual area of the segmentation mask is very small and after training, it is giving a lot of false positives. I am thinking of...

20 September 2020 1,968 2 View

How to model hysteresis property of concrete in abaqus?

I want to model rc element subjected to cyclic loading, How do I enter hysteresis data in the material model? I want to use Mander's 1988 model to get data for concrete. I dont know how to enter...

06 September 2020 7,651 3 View

Which fractional operator (with singular/nonsingular kernels, local, discrete, etc.) is more effective for modeling biological systems/processes?

To identify the most important fractional operator which is physically significant for the modeling of biological systems/processes (Viral dynamics, Fluid dynamics, Calcium dynamics and so on) to...

08 July 2020 8,146 3 View

Which one is the best method for bifurcation analysis in fractional-order epidemic models?

I want to know which one is the best method for obtaining bifurcation analysis in different types of fractional-order epidemic models.Any material suggestion is highly appreciated.

04 April 2020 784 3 View

How to Integrate of Sumo and Veins?

Hi everyone, I have installed omnet 4.3, sumo 0.21.0 and veins 3.0 (This version was necessary as I want to work with NETA framework) . I was able to run the example from Veins and also I...

07 January 2020 2,824 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View