Daniel Ferreira Feijó

3 Questions 5 Answers 0 Followers

Questions related from Daniel Ferreira Feijó

How do you disrupt interaction among dendritic cells from T cells?

I am having problems in my sortings of CD8+ DC from spleens of chronically infected mice. I did a gene expression array and, after analysis, I identified a lot of T cell markers (CD3e, Thy1, CD8b,...

05 March 2014 3,996 5 View

Does anyone have a protocol for generating dendritic cells from a mouse spleen using GM-CSF?

I would like to make and characterize dendritic cells derived from a mouse spleen.

20 February 2014 748 1 View

Does anyone know a good protocol to generate OVA peptide-pulsed dendritic cells (DC) in vitro?

Hello, I would like to know if anyone has a good protocol how to generate pulsed (DC) in vitro. I have some doubts about to maximize the procedure: 1) Are bone marrow derived DC better to be...

12 February 2014 733 11 View