Daniel Ferreira Feijó

4 Questions 5 Answers 0 Followers

Questions related from Daniel Ferreira Feijó

Is there any protocol to test interaction between dendritic cell and platelets?

Hi. I am looking for a protocol to do an assay about the interaction between dendritic cells and platelets in a murine model. Questions of kind such as: proportion of dendritic: platelet/well?...

14 November 2014 8,094 1 View

How do you disrupt interaction among dendritic cells from T cells?

I am having problems in my sortings of CD8+ DC from spleens of chronically infected mice. I did a gene expression array and, after analysis, I identified a lot of T cell markers (CD3e, Thy1, CD8b,...

05 March 2014 4,059 5 View

Does anyone have a protocol for generating dendritic cells from a mouse spleen using GM-CSF?

I would like to make and characterize dendritic cells derived from a mouse spleen.

20 February 2014 793 1 View

Does anyone know a good protocol to generate OVA peptide-pulsed dendritic cells (DC) in vitro?

Hello, I would like to know if anyone has a good protocol how to generate pulsed (DC) in vitro. I have some doubts about to maximize the procedure: 1) Are bone marrow derived DC better to be...

12 February 2014 780 11 View