After time lapse imaging of live cells, I need to track single cells to analyze motility from a series of bright field images in stack. Looking for a software which does that. I found a plug in called Trackmate in Fiji (an extended ImgaeJ version) but wonder whether that plug in can track cells in images other than those obtained from fluorescence imaging.
It should work fine on the negatives of your images as it recognizes light objects against dark backgrounds.
Brightfield is a difficult modality for cell tracking. Fluorescence is much more robust. You should definitely avoid DIC and try to get the most uniform illumination possible. CellTrack is the only program I have found to repeatably segment transmitted light images: http://bio.cse.ohio-state.edu/CellTrack/.
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