How is the width and depth of a neural network in machine learning selected for optimal processing?

How is the depth and width of a neural network in machine learning chosen for optimal processing? Given that neural networks are tending to be deeper now (thanks to more efficient coding and...

25 January 2021 9,904 3 View

Italicization in molecule name: N-acetyl-p-benzoquinoneimine (NAPQI)?

Is there a convention for correct italicization of abbreviated parts of chemical molecule names? For...

13 January 2021 7,396 3 View

Are there definitive rules or conditions for the creation of a quantum mechanically entangled state?

Are there some (simple) rules or conditions which dictate whether a physical process will lead to a state of quantum entanglement of two or more particles or systems? Given the relevance of the...

13 December 2020 7,590 3 View

Recommendation for Finding (Ocean Warming) Datasets?

Hello! I'm having trouble finding a suitable dataset that I'm pretty sure must exist. I've spent a couple days on Google without luck. If anyone has any dataset search websites to recommend please...

24 June 2020 2,008 7 View

Why do US National Burn Repository cases with valid hospital charges data have longer mean hospital days per %TBSA compared with survivors overall?

Successive National Burn Repository (NBR) Annual Reports consistently show a mean of approximately 1 hospital day per %TBSA burn across all patient records. For example, analyzing data from Table...

24 February 2020 489 2 View

What is the difference between Cyclic Voltammetry(CV) and Galvanostatic Charge-Discharge (GCD)??

What is the difference between Cyclic Voltammetry(CV) and Galvanostatic Charge-Discharge (GCD) especially when the samples to be run are supercapacitor or electrode material?? What is their...

11 March 2019 990 8 View

How big of an fold change value is possible to measure with qRT-PCR?

How big of a relative fold change in RNA expression can I reasonably expect to be able to quantitate using qRT-PCR? I have a highly expressed gene that I'm knocking down, and I know (via a...

19 November 2018 6,875 2 View

Might Bose-Einstein condensates be a means to create black holes?

If its possible to create a Bose-Einstein condensate for which no possible Fermi interactions can prevent further collapse and the mass was large enough, could the system collapse into a black...

02 December 2017 2,664 12 View

Why do i have late amplification of my gene in qPCR even if i add more cDNA

25 July 2017 887 6 View

How to calculate cDNA concentration after reverse transcription ?

11 July 2017 8,922 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View