I am doing an in situ hybridization and have localised my expression to the developing zebrafish eye. I would like to have some kind of positive control, but am not too familiar with eye development in the first 5 dpf. Is there a gene that is expressed symmetrically during zebrafish eye development?
Thank you!
Dear Monika,
probably the best canbdidate would be Pax-6, but you can try as well shh and otx-2.
Good luck
Dear Monica, You can also try with cadm3, atoh7, cdkn1ca and ccnd1 .
Good luck
Lots of genes are expressed during eye development, as above. If you want something REALLY specific to the eye, try crx (for photoreceptor) or cryaa (for lens). Are there particular parts of the eye you're looking for (lens, retina, CMZ, RPE) or specific cell populations?
This paper from our lab has quite a few good eye markers:
Uribe, R. a and Gross, J. M. (2010). Id2a influences neuron and glia formation in the zebrafish retina by modulating retinoblast cell cycle kinetics. Development 137, 3763–74.
hi Monika,
as colleagues mentioned above, Pax6 sounds like a good option. As a transcription factor, Pax6 is one of the earliest genes expressed in the eye and regulates the expression of many genes required for retinal and for olfactory development (PMID: 10461206). Also due to its key role as a master regulator in eye development, mutations in Pax6 have been associated with many different eye diseases.
Rax1 (also called rx1) + Pax6 should answer to your question.
Please, check here (from zfin)
https://zfin.org/ZDB-FIG-060926-11
Enjoy
P
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