We use two different tubes to obtain a buffy coat from human samples -- one containing citrate and one containing heparin, so both yield a plasma layer. Is there a way to reverse the anti-coagulation and derive serum from this plasma?
Meaghan,
Your question is very interesting. First of all let me explain to you the difference between plasma and serum. Plasma is obtained from blood which has been anticoagulated with either sodium citrate, hirudin or heparin or any other anticoagulant when it is centrifuged at 3000 rpm for 20 minutes. Serum is obtained from clotted blood after centrifugation at 3000 rpm for 20 minutes.
Now your question:
"Is there a way to obtain serum from plasma that has been anticoaguted with an anticoagulant?". The answer is YES. You will have to put higher concentrations of calcium chloride. You can also put higher concentrations of Thrombin or higher concentrations of tissue factor for that matter, Once you make a clot, the clotted sample may be centrifuged at 3000 rpm for 20 minutes to obtain, serum as a supernatant. You may also use anticoagulated blood to make the serum following this technique. I hope this helps.
.
I will try both calcium chloride and thrombin. Thank you for your advice -- this has been a great help.
Just by elimination of fibrinogen from plsma you may obtain serum.How?Deactivation of fibrinogen and conversion it to fibrin by addition of thrombin anf centrifigation of sample.
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