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Questions related from Meaghan Roy-O'Reilly
I know the reverse is possible, just wondering if anyone has any experience or literature they can recommend on male to female bone marrow chimerism. Thank you!
04 May 2015 9,278 6 View
We're studying differential miRNA expression 24 hours after injury in human clinical samples. What are the advantages/disadvantages of using whole blood (cellular + extracellular) instead of serum...
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Looking to compare several methods -- any advice appreciated!
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I'm currently developing a human flow panel for peripheral blood cells. The lymphocyte and monocyte sub-populations (CD4, CD8, CD20, CD14, CD16) look as expected, but although we are getting a...
22 October 2014 344 7 View
We use two different tubes to obtain a buffy coat from human samples -- one containing citrate and one containing heparin, so both yield a plasma layer. Is there a way to reverse the...
09 October 2014 4,874 3 View
I've seen a few different recipes for staining/washing buffers used in flow cytometry of human PBMCs and neutrophils, including PBS with 2% BSA and PBS with 0.5% BSA and 0.03% NaN3. We fix our...
26 September 2014 401 10 View
I'm in the process of designing a simple flow panel for human PBMCs and am looking for one marker that would best identify neutrophils. CD66b seems like a relatively new and specific marker, but...
22 September 2014 889 6 View