Meaghan Roy-O'Reilly

9 Questions 17 Answers 0 Followers

Questions related from Meaghan Roy-O'Reilly

Where do you obtain healthy control samples for your research?

While our lab has a significant amount of disease-associated patient blood samples, we do not have access to many healthy controls. If your work involves healthy control samples, where do you...

03 December 2017 6,374 1 View

How do I explore novel transcripts identified during RNA sequencing?

My lab recently conducted an RNA sequencing experiment examining differentially expressed genes between a control group and an injury group. At this point, we're just using Illumina BaseSpace to...

08 May 2015 190 3 View

Has anyone successfully transplanted male bone marrow into female recipients in mice?

I know the reverse is possible, just wondering if anyone has any experience or literature they can recommend on male to female bone marrow chimerism. Thank you!

04 May 2015 9,328 6 View

What is the best method of microRNA extraction?

We're studying differential miRNA expression 24 hours after injury in human clinical samples. What are the advantages/disadvantages of using whole blood (cellular + extracellular) instead of serum...

30 April 2015 3,275 3 View

Can anyone recommend a good protocol for flow cytometry analysis of granulocytes from fresh human blood?

Looking to compare several methods -- any advice appreciated!

11 November 2014 8,104 4 View

Loosing CD16+ granulocytes in human peripheral blood panel for flow cytometry?

I'm currently developing a human flow panel for peripheral blood cells. The lymphocyte and monocyte sub-populations (CD4, CD8, CD20, CD14, CD16) look as expected, but although we are getting a...

22 October 2014 392 7 View

Is there a way to obtain serum from plasma that has been treated with anti-coagulant?

We use two different tubes to obtain a buffy coat from human samples -- one containing citrate and one containing heparin, so both yield a plasma layer. Is there a way to reverse the...

09 October 2014 4,915 3 View

What buffer do you use for flow cytometric analysis of human blood cells?

I've seen a few different recipes for staining/washing buffers used in flow cytometry of human PBMCs and neutrophils, including PBS with 2% BSA and PBS with 0.5% BSA and 0.03% NaN3. We fix our...

26 September 2014 462 10 View

What is the single best marker for human neutrophils?

I'm in the process of designing a simple flow panel for human PBMCs and am looking for one marker that would best identify neutrophils. CD66b seems like a relatively new and specific marker, but...

22 September 2014 928 6 View