If you own agricultural land, would you use natural practices or rather you will use pesticides and chemical fertilizers to increase crop's yields?

By being aware of the harmful effects of all that is fertilizers and chemical pesticides on the soil, the environment and the health and if one day we would be owners of agricultural land, how...

21 December 2020 9,899 23 View

Can you please complete an onlinse survey? (only if you are 18 or over and you consume herbal tea or tisans)?

Hello, I am a research student in MSc Food Science and Innovation at Manchester Metropolitan University for 2019-2020. I would hereby like to invite you to take part in my dissertation research...

30 November 2020 528 2 View

Is there free software available to model and simulate a membrane bioreactor?

I'm looking for a free software to model and simulate a membrane bioreactor (MBR).

29 November 2020 8,385 3 View

Alternative of potassium antimonyl tartrate?

What is the alternative of potassium antimonyl tartrate that can we add in the reaction for the detection of phosphate in the molybdenum blue method (Murphy and Riley, 1962) ?

19 September 2016 6,257 10 View

How can we determine the production of hydrogen cyanide ?

Usually , the moethod used for the determination HCN is based on picric acid, but according to it explosive and toxicity proporties, we prevent to use it. Is there any other method to determine...

07 September 2016 369 7 View

Petroleum analysis with GC-FID or FITR ?

26 August 2016 9,282 2 View

What is the best manner to theoretically model the ionic liquid effects on Diels Alder reactions?

I need some information on the best manner to study the effects of ionic liquids, like a solvent, on DA reaction.

25 February 2013 8,097 2 View

What is the procedure for making a Monte Carlo simulation of solvent effects?

I want to doing a simulation of the solvent effect on Diels-Alder reactions with the Monte Carlo method but I need some help to understand this method (theory) and the procedure.

05 June 2012 7,921 5 View

Difference between resistance and tolerance

What is the difference between resistance and tolerance to a compound? If there is bacteria that grow in a indirect contact of a toxic compound, when can we say if this bacteria resist or...

01 January 1970 6,818 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View