I am a masters student and have a presentation of theoretical applications of regenerative medicine. I need a xenoenzyme to be exocytosed from an endothelial progenitor cell. I thought there would be a know tag/ sequence that could be incorporated to tell the cell to export the protein but I have had no luck finding any. How would you go about ensuring this protein is secreted by the cell?
Any help is much appreciated. Thanks!
Dear Stephen,
I think that TTSS (T3SS, Injectisome or Injectosome) a protein appendage found in several Gram-negative bacteria can be a good example.
For details please use the following link:
https://en.wikipedia.org/wiki/Type_three_secretion_system
Hoping this will be helpful,
Rafik
I think you should add at the N-terminus of your enzyme a pre-pro sequence, similar to those of released hormones or growth factors