Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000?

Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000? If yes- Is there some maximum number of bp of RNA, which could be transfected using Lipofectamine 3000? I...

08 November 2018 9,558 3 View

What is the optimal concentration of miRNA for microinjection into porcine oocytes?

Our stock is 5nmol. Or is it different for each miRNA? Thank you for answers.

30 May 2018 6,655 2 View

Someone who is able to statisticaly analyze MALDI-TOF data?

I am looking for anyone with experience with statistical comparison of MALDI-TOF data. I have no experience with analyzing profile data and I need to compare the effect of the treatment on...

19 September 2017 5,091 3 View

How to define multi volume heat fluxes within DFLUX subroutine, Abaqus?

24 May 2017 1,673 1 View

Why reporters of deviations in pharmacotherapy are not established and well paid in many European hospitals?

According to the media many fatal medical errors have been happened in many European countries. Although a 'soul' of inappropriate treatment and prescribing is well known in the literature, in a...

31 May 2016 2,834 8 View

Why and when should we combine or switch antidepressant in patients with major depressive disorder MDD?

02 January 2016 8,597 18 View

Why many Eastern European countries did not establish clinical pharmacy service in the hospitals next to the patients? Who will deal with errors??

According to the newest evidence more than 10 % of patients are admitted to the hospital, because of inappropriate prescribing and drug-related problems. More than 20 % of patients are treated...

11 October 2015 8,973 57 View

What is your opinion about quality of antibiotic prescribing within psychiatric wards and hospitals?

Prudent prescribing of antimicrobial drugs to hospital inpatients may reduce the incidence of antimicrobial drug resistance and healthcare-associated infection. Despite strenuous efforts to...

24 March 2015 3,714 14 View

Are there any studies showing age related hypertension in regular strains of mice?

In particular I'm interested if hypertension occurs in C57BL6 mice at any time after their 18th month of age. But I am also interested in other strains. If you could point me to such studies I...

04 March 2015 401 6 View

Can anyone offer us analysis of homoarginine?

We have introduced the methodology for the estimation of reactive lysine in our laboratory. In the last step determination of homoarginine is necessary. Can anyone offer us this analysis?

01 February 2015 9,714 4 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View