Is there a table that shows the codon usage probability for earthworm species?

18 April 2023 1 9K Report

If not, which earthworm-related species or animals (particularly E. Fetida) should be used?

this Japanese site is very useful, sometimes offering a related species if not the exact one needed, https://www.kazusa.or.jp/codon/

good luck!

Badges
Science topic

More Nima Mohseni's questions See All

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,688 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,288 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,781 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,331 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,082 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

Should I put stop codon on light chain to form Fab?

If I want to put mouse light chain(Sac1 and XbaI) and heavy chain (XhoI and SpeI) in pComb3XSS (phagemid) to form Fab, should I add stop codon at the light chain?

20 May 2024 806 0 View

[LC-HRMS] Mass higher than normal for Intrinsicaly disordered domain?

For my project, I am attempting to purify an intrinsically disordered domain cloned in a pET28b vector. The strain used for overexpression is Rosetta II(DE3). Firstly, I encountered an unexpected...

23 April 2024 9,938 4 View

How to submit coding sequences in genebank?

Hii all.. I have few sequences of Fusarium spp partial TEF region. I want to submit them to genebank to get accession numbers. Can someone please help how to submit sequences of coding regions? It...

23 April 2024 2,468 6 View

In gene bank submission, what is the reason of "your seq. contains stop codons" ?

I am trying to submit sequences of COX-1 gene(mitochondrial gene) to gene bank. My sequences is almost 100% identical to a published one. It return a message saying that they contain stop codons...

12 April 2024 3,503 0 View

Substitution matrix for codon (molecular evolution) ?

Hello, Does anyone knows how to get actual substitution matrices for codon ? I can get the amino-acid substitution matrices through the GiPhy website...

24 March 2024 5,614 0 View

Multiple primer sequences in sequencing result after PCR?

I was doing site-directed mutagenesis using PCR. And I have ordered forward and reverse primers that are reverse complements of each other (both primers are around 45bp, 7 aa before and after my...

19 March 2024 5,082 0 View

What should be the gc ratio of the genes to be expressed for pichia pastoris?

I need to know that after codon optimization what should be the GC content?

04 March 2024 7,074 1 View

Reference mapping and de novo algorithm for genome assembly?

Hello, I am working on viral metagenomics and have started using the High Throughput Sequencing reads for virus genome assembly. Since, I am at the beginner level, I have some confusions. As these...

01 February 2024 296 1 View

Cloning with pAcGP67A vector with GP64A signal peptide?

Hello everyone, Is there anyone who has cloned with pAcGP67A secretion vector with GP67A signal peptide. I tried to clone with insert 1.2kb in the vector, MCS: BamHI and NotI. C-term Thrombin and...

12 December 2023 5,090 1 View

Bacteriocin expression in pET-19b?

I am looking for vectors for expression of bacteriocin, and from pET series I think that 19b fits the most, I am just having few questions. When I clone my insert in NdeI and XhoI, i will have...

12 December 2023 2,321 5 View