If I want to put mouse light chain(Sac1 and XbaI) and heavy chain (XhoI and SpeI) in pComb3XSS (phagemid) to form Fab, should I add stop codon at the light chain?
What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?
30 July 2024 7,376 3 View
After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?
29 July 2024 4,955 0 View
all math can be traversed by code? all math can be translate to code?
26 July 2024 9,530 0 View
The system matrix has 172 negative eigenvalues. Displacement increment for contact is too big. Displacement increment for contact is too big. The strain increment has exceeded fifty times the...
05 July 2024 3,839 2 View
What is the effect for the CTR model, adding the tag-id with the highest number of user clicks/purchases on the item's tag as user-side features?
22 June 2024 3,588 1 View
Greetings all, My group has been trying to reproduce 3t3-L1 cell (preadipocytes) differentiation. The typical protocol involves 3 steps: the first step and second step both last for 2 days, and...
18 June 2024 2,728 1 View
Hi, I am trying to make synthetic phage library. Right now , do people still use Kunkel method or other methods? Since it looks like Kunkel method is an old method. I am looking for more...
09 June 2024 6,780 1 View
The primary problem that large language models solved is small sample learning, right?
03 June 2024 3,305 2 View
based on GPT-3
01 June 2024 5,445 3 View
Or they are only complement to each other
01 June 2024 6,021 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View