How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View

How many authors?

Hello, I'm currently finalizing my Ph.D. proposal in Research in English Literature. I still have to apply to universities abroad. My question is can I pick two authors for my research (for...

08 February 2021 6,858 12 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How can sea water be used as an input for green hydrogen production?

If you extract sea water, and filter it, would it be suitable as an input for green hydrogen production? From an electrochemical perspective, would this be the only process step required?

13 December 2020 2,665 8 View

Common marker for B and T lymphocytes?

I have to identify lymphocytes on histological sections (after PFA fixation). Please is there any good marker which can be used to label both B and T lymphocytes? Thanks

03 December 2020 1,291 7 View

Are there any interesting areas of research yet to be studied with rice viruses?

I'm currently in the process of coming up with a research pitch for a lab to be funded here in the Philippines. Rice is quite the common crop in the South East and its diseases are commonplace....

23 November 2020 5,194 4 View

How do I extract values from polygons or points from a NetCDF layer file for specific geographical locations at specific points in time?

I have a NetCDF layer file with monthly SPEI-6 values between the years 1950 and 2020 from Global Drought Monitor, a shapefile with polygons of geographical areas that I want to use for extracting...

16 November 2020 2,739 7 View

List of peptide structure prediction services?

Hi all, I am interested in peptide structure prediction services, both secondary structure and tertiary structure. Protein prediction services that can predict structures of short peptides are...

30 October 2020 4,314 4 View

Reviews on Social Organization in Rodents?

Looking for papers which detail the various modes of social organization in rodent species.

10 October 2020 3,654 3 View

Should I consider the internal resistance only R0 or should I calculate the whole resistance of the circuit?

Hey! So I'm trying to find an internal resistance of a cell battery (Li-Ion) So, basically what they have given me: A battery open circuit voltage connected in series with a resistor (R0) and...

07 October 2020 6,693 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View