For example, adjust settings like time, duty, power, temperature, and so on in 2-mL, 3-mL microtubes, Falcons, etc. that contain the plant and extraction buffer for measurement enzymes, soluble protein, etc.
Hallo!
Please look at: https://bandelin.com/prospekte/Labor_SONOPULS_Prospekt_DE_BANDELIN.pdf .
Best regards
Stefan Bandelin
Sorry, the english Version is: https://bandelin.com/prospekte/Laboratory_SONOPULS_Brochure_GB_BANDELIN.pdf
No, there is not a specific protocol for general use, since every application is different. Your protocol is going to depend on your instrument, probe, sample type, sample volume, and desired outcome.
You can find more information on homogenizations applications and protocols here: https://homogenizers.net/pages/ac-application-center ... But please provide more information on your application and myself and others will be better able to provide guidance.
Dear Stefan Bandelin & Carlton Hoyt
Thank you very much for your guidance. I plan to set up this type of homogenizer in my lab for the first time. However, I did not have a clear protocol for how it works. My current settings caused the materials to be ejected from my microtube.
Dear Faride Noroozi Shahri - You are setting the amplitude too high on the ultrasonic homogenizer and / or filling the tube too much. When processing in a microtube, you do not want it to be more than half full, preferably a bit less (but I recommend at least 200 µl volume). If your volume is greater than 1/2 the volume of your microtube, you may want to consider using a larger vessel, such as a 5 ml tube. Start the ultrasonic homogenization process using the lowest amplitude setting your device supports, then slowly increase it.
Also, ensure you are not using a probe more than about 3 mm. Anything larger would be too large for processing in microtubes.
Hope that helps!
- Badges
- Science topic
- Similar topics
- Computer Communications (Networks)
- Simulators