Does SWAT model not support high resolution gridded climate (0.25 degree) data?

Dear all, I have prepared rainfall datasets for SWAT MODEL using 0.25 degree IMD long term gridded rainfall data, but as I am using it in the model, it is rounding off the co-ordinates...

03 October 2020 421 5 View

How to separate mixture of two organic compounds using size exclusion chromatography?

My organic compounds have a molecular weight in the range of 2500g/mol and other is 1066.22 g/mol. aThe TLC results show three spots. But the main problem with my compounds are acid sensitivity of...

20 August 2020 6,119 3 View

Monolitic construction of brick masonary and concrete simultaniously have any effect ?

Concrete column casting after masonary completed monolithic.

17 August 2020 7,172 3 View

Rentraint Corner of building if need extra stitching diagonal reinforcement ?

If temperature reinforcement already provided ,at reentraint corner need extra diagonal reinforcement ,if needed then what amount of steel.

12 August 2020 1,707 2 View

Does more number of citations of any article and higher the impact factor of any journal reflects the quality of work??

i.e., Are they dependent??

08 June 2020 8,062 4 View

Could I use ELISA kit for MUC 1 to measure the CA 15-3 ?

I currently doing a research for anticancer drugs and have a plan to use CA 15-3 as tumor marker to measure the effectivity. Some reference tell that CA 15-3 and MUC 1 are synonym, but i also...

10 September 2019 2,125 5 View

Can any explain me the mechanism of phosphomolebdnum reduction Assay?

Why we are boiling the sample at 95 c and for 90 min

22 May 2019 1,286 2 View

How to get rid of reflection light from my experiment( A moving object )- Dynamic DIC ?

I am working on image processing getting through captured video from high speed camera but due to motion of target object, there is reflection of light on the some videos images. I used 2 high...

21 May 2019 5,020 4 View

Can we calculate surface roughness from RGB/Gray scale images?

I am interested in measurement of the surface roughness through stereo photo images. Any reading suggestion/direction is highly appreciable. Thanks in advance; Abhilash Singh

19 April 2019 7,434 1 View

How TSN over Ethernet can be integrated with AI in order to compute the adequate traffic scheduling for the network?

TSN Ethernet Integration Artificial Intelligence Switches To compute the adequate traffic scheduling For the network?

21 March 2019 3,683 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View