In our lab we use dsRNA to screen plant material for the presence of viral RNA. But however I have been getting inconsistent results. TRIZOL hasnt realy worked for me since it isolates Total RNA so am in a bit of a quagmire
I had used also RNeasy Mini Kit for isolation from plant tissues and it worked very well!
Hi Tichaona,
Have a look at these articles. dsRNA isolation is a delicate experiment. since you're dealing with RNA, make sure that you're working in a RNAse free environment.
Article Phylogenomic Analysis Reveals Deep Divergence and Recombinat...
Tichaona,
We use the RNeasy Mini Kit (see link below) for RNA isolation and it works quite well. I used to do TRIzol isolations, but the kit seems to give better results. I am not sure what your lab's funding situation is, but it may be worth the cost to save yourself the hassle. Best of luck.
https://www.qiagen.com/us/products/catalog/sample-technologies/rna-sample-technologies/total-rna/rneasy-mini-kit
I had used also RNeasy Mini Kit for isolation from plant tissues and it worked very well!
Thank you for the responses.
@Christopher and @Smirnova well our budget is very limited. Its a service we offer to farmers so a kit will mean Higher cost...which farmers wont like at all...so it has to be "in house"
@Jacques Thanks a lot for the publications. perusing them now as I plan my next move. very informative.
Again Thank you folks!!
Smirnova Anna @christopher but RNeasy Mini Kit isolates total RNA. How did you separate dsRNA from total RNA?
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