How do I extract pure colonies of Sclerotinia sclerotiorum from fresh tobacco leaves for the purposes of DNA extraction

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How can I use Image J software to measure thickness of Intestine mucosa and Villi?

In particular the Villi from a cross section of mouse intestine

03 April 2017 1,551 0 View

Is there a simpler method for dsRNA isolation besides the TRIZOL method?

In our lab we use dsRNA to screen plant material for the presence of viral RNA. But however I have been getting inconsistent results. TRIZOL hasnt realy worked for me since it isolates Total RNA...

05 June 2015 4,021 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Claire MM Gachon

using standard microbiology techniques. One example among many would be: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769566/

Once you have isolates on agar plates, it is easy to purify them down to clonality by streaking a diluted spore suspension (or mycelium fragments) on a plate, and pick individual colonies.

Ajay Gautam

Preparation and maintenance of pure culture by spore suspension or mycelium fragments is of prime importance. then you can use mycelium form this culture for DNA extraction.