I need to separate IgM, IgG and IgA from blood serum by a simple method/ kit.
Use protin G- sepharse to capture the IgGs; you can do it by pouring serum over a column or by addition, mixing and centrifugation. The different isotopes elute from the washed sepharose at different pH's.
check the literature...
Thank you for your answer.
see e.g. these resins for alternatives
http://www.lifetechnologies.com/de/de/home/life-science/bioproduction/poros-chromatography-resin/research-scale-purification/antibody-purification.html
Thank you, Markus
Below paper might help...
http://www.bioorganica.org.ua/UBAdenovo/pubs_3_2_05/Nikolayenko.pdf
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