Shailesh Parab

4 Questions 10 Answers 0 Followers

Questions related from Shailesh Parab

Can anyone suggest stabilizers for antibody conjugated to HRP polymer?

I have prepared HRP polymer with periodate method and conjugated the so formed polymer with antibody. But I find difficult to stabilize the same... Can anyone help?

03 March 2015 7,702 7 View

How to evaluate ELISA reader or Luminescent Reader?

I want to know how we can evaluate ELISA reader or Luminescent Reader so as to check its reliability and accuracy.

12 December 2014 4,617 3 View

How can I adsorb Dextran to colloidal gold (prepared as per Frens et al., 1973)?

I want to adsorb dextran to colloidal gold for preparation of monodisperse colloidal suspension.

05 May 2014 7,305 6 View

How to tackle with naturally occuring anti-streptavidin, which are interfering in Immunoassay?

I am working in developing ELISA for anti-HCV detection. I am using biotinylated peptides as a capture on streptavidin coated plates. My detection system is protein-A conjugated to HRPO. Now I...

01 January 1970 9,474 1 View