Dear Sir. Concerning your issue about a simple method to dose glycolipids (cerebrosides) like alpha-galactosylceramide. A two-dose protocol, with doses being administered 1 day before and 1 day after infection, gives the best protective effect. Glycolipid C34 (A) or 7DW8-5 (B) was administered to 7-week-old C57BL/6 mice by a two-dose protocol, as described in the legend. For more details, I think the following below links may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2944612/

http://www.scielo.org.co/pdf/vitae/v22n1/v22n1a2.pdf

http://www.lipidhome.co.uk/lipids/sphingo/cmh/index.htm

Thanks

Dear Isam, thanks for your help.

However, I am still in doubt if there is a method to quantify the amount of cerebrosides in a solution.

Best regards