This is for a research protocol in spider monkeys' chronobiology. So far the best saliva extracting device has been an aspirator that allows collecting about 1 ml in 2-8 min. The issue is that most monkeys are unwilling to cooperate if they don't receive a food reward while aspirating the saliva, thus contaminating the sample with the treats used to lure them.
The three analytes will be quantified by ELISA, nevertheless, if someone knew of a similar assay less prone to disturbance by the use of salivary stimulants it would be of great assistance as well. Thanks to anyone that can help me in this matter.