Have anyone tried SYBR green with GoTaq DNA Polymerase for a qRT-PCR?

Hi, everyone. I need to perform a real time reverse-transcription PCR in one step. What we have is SYBR green I PCR Master mix with GoTaq DNA Polymerase. So far, our attemps to perform the assay...

07 January 2021 6,507 3 View

Does international survey based researches needs approval in each country ethics commitee?

We plan a contingent valuation of ecosystem services. The study will be based on surveys. We want to apply it in fifteen different countries. Is it necessary to approve the project in ethics...

25 June 2020 8,099 3 View

How to choose the best smooth algorithm for your data in Origin?

I have several curves of applied potential versus electric current with hundreds of points in each of them. I am trying to analyze their first and second derivatives, but they are too noisy to...

27 April 2020 5,932 2 View

I can't make my RAPD-PCR work anymore. Can anyone help me?

Guys, I REALLY NEED HELP At the end of the last year I optimized every parameter of my RAPD-PCR, and as a result I did obtain great products multiple times. Then, I went home for my vacation (I...

18 February 2020 9,236 3 View

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10 December 2019 7,354 5 View

Hi, I would like to solubilize a hidrophobic compound in order to administer it to mices. Should anyone suggest a nontoxic vehicle?

The compound is very insoluble in aqueous solution and the compound administration will be given orally. The compound manufacturer has no information regarding the solubility of the compound....

16 January 2019 834 1 View

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Hello everyone! I have been consulting this webpage http://beast.community/ and http://www.beast2.org/ , but the examples for the use of BEAUti and BEAST are for old versions. The versions of...

02 April 2018 3,942 3 View

Two-step cluster in SPSS - how to validate results?

Hi everybody. After applying a two-step cluster in SPSS, involving both continuous and nominal variables, how can I validate if the results are satisfactory? In other clustering methods there are...

03 October 2017 5,765 8 View

Have you COI sequences of Potamotrygon orbigyi?

24 August 2017 6,634 2 View

How many sequences it needed to put in a crown group to calibrate a tree?

17 August 2017 4,155 2 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View