I'm searching for a relation between agarose gel concentration and pore size. Also, could it reach a pore size of 1000 nm?
In the lab, differing concentrations of agarose gel are used depending on the size of the DNA fragments being studied - if in general the DNA fragments used are small, then a more concentrated gel should be used. Porosity is a measure of how many pores there are in a given agarose gel - with a greater gel concentration, porosity decreases. Pore size distribution is the set of the sizes of pores that exist in the gel - in a more concentrated gel, the largest pore that exists is likely to be smaller than in a less concentrated gel. Agarose gel has large pore size and good gel strength, making it suitable as an anticonvection medium for the electrophoresis of DNA and large protein molecules. The pore size of a 1% gel has been estimated from 100 nm to 200-500 nm.
Both, pore size and number could be affected by agarose gel concentration. This is the way that agarose gel can separate the DNA fragments with different size.
Thank you Dr. Leabaneng, Dr. Ayoob for sharing this information
Thanks Dr. Ali,
It's mentioned in some papers that the concentration of the buffer can affect the pore size.
My aim is to reach a pore size of 1000 nm. Do you have an idea of how to reach this?
Simply... there is an inverse relationship between gel concentration and pore's size.
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Higher concentration of agarose gel for smaller size of DNA molecules. eg:- .08% agarose gel for large Dna molecules and vise versa 1% or 2% agarose gel for smaller DNA size.
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