It's descried in most papers that NTA is performed using NanoSight (ex NS300, Malvern). Actually, we don't have such a device. Instead, we have a zetasizer device. Will it work or not??
This depends on your sample. There are actually quite a few referenced publications of exosome data with the Zetasizer.
http://www.materials-talks.com/blog/2014/11/17/tips-tricks-for-nanoparticle-characterization/#exosomes
Hi Abdulaziz,
You may be able to use a zetasizer device (DLS) to determine the size distribution of your exosome sample. Attached is an article which demonstrates the use of DLS to characterise exosomes from a colorectal cancer cell line (LIM1863).
Article Highly-purified exosomes and shed microvesicles isolated fro...
I have also attached a review article which highlights the advantages and disadvantages associated with using this technique
Article The Methods of Choice for Extracellular Vesicles (EVs) Chara...
Hope this helps.
Hi Abdulaziz,
As Mary and Ulf correctly state above you can use a Zetasizer for obtaining size of exosomes, but this will it will not be by the NTA-principle, that requires a different instrument with a completely different layout.
I would prefer doing size analysis of these samples by Zetasizer. I know the NTA method is popular among many R&D groups, but as a colloidchemist I have a strong preference for DLS (meaning a Zetasizer or similar). For now I am still sceptical about the NTA principle.
Kind regards, Leo
- Badges
- Science topic
- Similar topics
- Chemistry
- Nanotechnology
- Nanostructured Materials
- Nanoparticles