12 December 2016 3 8K Report

I need recommendations for a bioinformatics book starts from the very beginning and take the reader to a reasonable depth in the different concepts of bioinformatics.

It will be better if it has some statistics and programming concepts needed in bioinformatics research.

More Abdulaziz Ashraf Elgammal's questions See All
Can I perform nanoparticle tracking analysis (NTA) of exosomes using a zetasizer device?

It's descried in most papers that NTA is performed using NanoSight (ex NS300, Malvern). Actually, we don't have such a device. Instead, we have a zetasizer device. Will it work or not??

09 October 2017 6,129 3 View

What is the specific signal or method to load a protein inside extracellular vesicles?

Is there an established method or protocol to load a specific protein inside extracellular vesicles or to integrate it in the vesicle membrane? 

08 September 2017 1,764 3 View

Is there a relation between pore size and agarose gel concentration?

I'm searching for a relation between agarose gel concentration and pore size. Also, could it reach a pore size of 1000 nm?

03 April 2017 1,978 7 View

How to fix mammospheres for fluorescence screening ?

I'm working on mammospheres suspended in the media. I have no enough count to perform flow cytometry. I'm working on PCR for CSCs marker genes as well as the fluorescent markers. The problem is...

04 May 2016 4,086 6 View

Where can I find a detailed protocol for performing PCR on mammospheres?

I want to characterize the mammospheres using conventional PCR. I have performed the experiment 3 times but every time I have a problem with the results. I need a detailed protocol for the steps...

04 May 2016 8,805 1 View

Number of breast cancer stem cells needed for Flow cytometry?

I'm growing mammospheres isolated from breast cancer patient and I want to Identify the the markers on the surface of breast cancer stem cells inside the sample. how many cells are needed and what...

02 March 2016 8,066 5 View

How to perform the cytoskeleton staining using the confocal microscope on non adherent mamosphers?

I'm culturing mamospheres cancer stem cells and they are floating in the media. How can I help their attachment to perform the cytoskeleton staining or if there is another way to do it?

01 February 2016 4,268 2 View

Single cells grow in the media contains mammospheres?

I'm growing mammospheres from breast cancer stem cells and there are single cells grow in the media. Also I noticed that the mammospheres are not regular circles and the largest diameter does not...

01 February 2016 3,813 3 View

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