Can I perform nanoparticle tracking analysis (NTA) of exosomes using a zetasizer device?

It's descried in most papers that NTA is performed using NanoSight (ex NS300, Malvern). Actually, we don't have such a device. Instead, we have a zetasizer device. Will it work or not??

09 October 2017 6,061 3 View

What is the specific signal or method to load a protein inside extracellular vesicles?

08 September 2017 1,684 3 View

Is there a relation between pore size and agarose gel concentration?

03 April 2017 1,877 7 View

How to fix mammospheres for fluorescence screening ?

I'm working on mammospheres suspended in the media. I have no enough count to perform flow cytometry. I'm working on PCR for CSCs marker genes as well as the fluorescent markers. The problem is...

04 May 2016 3,996 6 View

Where can I find a detailed protocol for performing PCR on mammospheres?

I want to characterize the mammospheres using conventional PCR. I have performed the experiment 3 times but every time I have a problem with the results. I need a detailed protocol for the steps...

04 May 2016 8,703 1 View

Number of breast cancer stem cells needed for Flow cytometry?

I'm growing mammospheres isolated from breast cancer patient and I want to Identify the the markers on the surface of breast cancer stem cells inside the sample. how many cells are needed and what...

02 March 2016 8,008 5 View

How to perform the cytoskeleton staining using the confocal microscope on non adherent mamosphers?

I'm culturing mamospheres cancer stem cells and they are floating in the media. How can I help their attachment to perform the cytoskeleton staining or if there is another way to do it?

01 February 2016 4,183 2 View

Single cells grow in the media contains mammospheres?

I'm growing mammospheres from breast cancer stem cells and there are single cells grow in the media. Also I noticed that the mammospheres are not regular circles and the largest diameter does not...

01 February 2016 3,750 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View