9 Questions 5 Answers 0 Followers
Questions related from Abdulaziz Ashraf Elgammal
It's descried in most papers that NTA is performed using NanoSight (ex NS300, Malvern). Actually, we don't have such a device. Instead, we have a zetasizer device. Will it work or not??
10 October 2017 6,098 3 View
Is there an established method or protocol to load a specific protein inside extracellular vesicles or to integrate it in the vesicle membrane?
09 September 2017 1,734 3 View
I'm searching for a relation between agarose gel concentration and pore size. Also, could it reach a pore size of 1000 nm?
04 April 2017 1,926 7 View
I need recommendations for a bioinformatics book starts from the very beginning and take the reader to a reasonable depth in the different concepts of bioinformatics. It will be better if it has...
12 December 2016 8,133 3 View
I'm working on mammospheres suspended in the media. I have no enough count to perform flow cytometry. I'm working on PCR for CSCs marker genes as well as the fluorescent markers. The problem is...
05 May 2016 4,050 6 View
I want to characterize the mammospheres using conventional PCR. I have performed the experiment 3 times but every time I have a problem with the results. I need a detailed protocol for the steps...
05 May 2016 8,764 1 View
I'm growing mammospheres isolated from breast cancer patient and I want to Identify the the markers on the surface of breast cancer stem cells inside the sample. how many cells are needed and what...
03 March 2016 8,041 5 View
I'm culturing mamospheres cancer stem cells and they are floating in the media. How can I help their attachment to perform the cytoskeleton staining or if there is another way to do it?
02 February 2016 4,221 2 View
I'm growing mammospheres from breast cancer stem cells and there are single cells grow in the media. Also I noticed that the mammospheres are not regular circles and the largest diameter does not...
02 February 2016 3,784 3 View
