What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Disappearing Coomassie Bands?

Suddenly my Coomassie staining has produced strange results (see photo attached). The past 8 gels all share the same trends: the bottom half of the gel fades out and is absent of any bands. I...

19 December 2020 3,619 1 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

Masters thesis subject - International humanitarian law/ International criminal law?

Hello I am a master's student and I'm searching for a current debate in the field of international humanitarian law or international criminal law. Is there somebody who can help me with this?...

08 November 2020 1,571 13 View

Is it possible that a IncX and IncF plasmid fused?

I recently performed plasmid extraction and nanopore sequencing. I have found what looks like a plasmid with replicon types IncX and IncF. Even with spAdes, canu, unicycler (illumina and nanopore...

29 September 2020 7,778 2 View

Can anyone tell me which species (or higher taxon) this is?

We found this small, maybe parasitic, crustacean in the North Sea offshore. The posterior body part is missing, but it looks quite characteristic. There is double-hooked rostrum, 2 antennae...

08 April 2020 6,842 9 View

How does hypertonic tissue appear in sliding filament theory & how do the number of myosin heads in a hypertonic area affect muscle strength?

30 March 2020 2,169 5 View

Can I use time as a predictor variable in a panel regression?

Is it possible to generate a new variable "phase" that is based on the time the participants were questioned? I generated this new variable by dividing the time into three phases (beginning,...

30 March 2020 3,756 6 View

Dielectric constants of organic:aqueous mixtures, is linear addition a valid approximation?

I would like to compute dielectric constants of organic:aqueous mixtures. Would linear addition of the dielectric constants of the pure solvents give reliable results? For example, for a volume...

26 March 2020 5,939 3 View

Which SPSS test should I use?

For a small project, I have to calculate a few things via SPSS. However, I am not sure which tests to use. I have multiple variables. Independent variable: categorical variable with two levels....

12 March 2020 7,188 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,306 3 View

How is it possible to rank variables using Likert scale?

Hello Everyone, I have a questionnaire of a 3-point Likert scale for an overall evaluation for a service, and several detailed attributes evaluation of that service. For example: Overall...

31 January 2021 9,612 8 View

How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How do I access Domestic Violence Attitude Scales or find them for free?

Hi, I'm a postgraduate student currently starting up a research project on attitudes towards domestic violence (DV). The study will aim to compare cultural differences between attitudes and...

31 December 2020 781 3 View

What is the best Quality of Life questionnaire for chronic patients?

Hello! I am looking for a SHORT questionnaire/scale to measure Quality of Life in chronic patients (e.g. fibromyalgia, or multiple sclerosis). Ideally, validated for English and Spanish speakers....

22 December 2020 9,962 4 View

Is it always mandatory to report and test the validity and reliability of a newly developed questionnaire?

Hello everyone! Currently, I am doing an observational, cross-sectional study on knowledge, atittude, and practice towards thalassemia in Indonesia. My area of interest is in population studies....

16 December 2020 3,725 16 View

May I use Minimal important difference (MID) in non-normal distribution?

MID is based on Standard deviation, consequently, I should not use it in non-normal distribution. Am I right? If yes, which test may replace MID?

13 December 2020 7,573 3 View

I would like to claim authorship of the article below but have been unable to do so?

Experiences of sleep hygiene education as an intervention for sleep problems in children with developmental disabilities : findings from an exploratory study

07 December 2020 3,607 3 View

How to do Factor analysis for extracting items - side by side column questions ?

Hi everyone, I have side by side questions in the questionnaire. There are total 29 questions. Each question has more than 11 items and three side by side columns to capture different contexts...

02 December 2020 5,499 3 View