I need some tips to learn how to preserve some macrofungi by lyophilization end resin embedding. Do you have any answer? Thank You
In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, how do the results of Bland-Altman plots and regression...
09 April 2024 7,249 1 View
For design a Modular Battery for Launchers I need to know the following information: 1. Which is the typical thermal environment of the launcher for the battery? 2. If it is foreseen a heat...
26 February 2024 644 0 View
Hello everyone, I'm currently having issues in trying to obtain an extracted DNA sample with 260/230 ratio values ranging from 2.0 to 2.2 for Nanopore downstream analysis. Right now after...
01 November 2023 5,609 0 View
We are analysing such dataset and it seems that such EIS measurements are not fully consistent. The phases are scattered and very limited in absolute value. The Magnitude values look a little...
02 March 2023 1,901 1 View
Teaching reading to primary learners who doesn't know how to read
09 February 2023 3,862 3 View
I have a pre-post study, three groups, and one covariate. I have conducted two contrasts (1st group vs. 2nd+3rd; 2nd vs 3rd). The independent group is multicategorical, the dependent variable...
02 September 2022 5,789 5 View
Hello, I need to stain in a complete way all the cell membrane of a zebrafish larvae around 6dpf. I approached bodipy, but this staining doesn't work in fixed larvae. Some of you have some ab,...
23 November 2021 7,924 2 View
Hi, I would like to know if someone knows the composition of the Binding Buffer from the Dead Cell Removal Kit from Miltenyi Biotec. Have you tried a home-made alternative?
15 November 2021 4,803 1 View
I plan to treat C57BL/6J mice with a Western-type high-fat, high sugar diet for 16 weeks to induce obesity and NAFLD. I also plan to study ex-vivo central insulin resistance (Western blot in the...
25 July 2021 1,432 1 View
Hi, I am looking for a scale to determine if participants are more prone to accept scientific or 'transcendental explanations to alternative therapies. My hypothesis is that if...
02 May 2021 8,471 5 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...
23 July 2024 1,672 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
https://en.wikipedia.org/wiki/Viscosity
21 July 2024 3,089 4 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View