Some techniques state it is better to dissect the female mouse dorsally others state to use the normal ventral dissection method.
Hello
i prefer the normal ventral dissection method , where the Female genital apparatus is excised by making a cut on the median body of the uterus close to the neck (see picture). From here, proceeding upwards, the mesometrium ligaments, that fix the organ to the posterior wall of the abdomen, are cut up to the level of the ovaries at the back.The investigator will then delicately cut those ligaments by which the ovaries are attached to the inferior poles of the kidneys, as well as from the mesovarium ligaments, with which the ovaries are joined to the posterior wall of the abdomen. Isolated organs are extracted and examined for shape, volume, consistency and obvious lesions.
Good Luck
After dissecting the ventral side (as shown by Saleh), you can see the female tract very clearly. Ovaries above the convoluted oviducts.
People do transfer early embryos/ eggs from dorsal lateral side sides into the oviducts of live mouse,
Interesting question, dear Sassine!
The answer would very much depend on the objective of the dissection.
I have always performed ventral dissection, through the same procedures as Saled, (whom I congratulate for the beautiful image !), because my objective was to study the vascular aspects of the female genital tract, and I start with the ventral dissection of the thoracic wall, to cannulate the Aorta, and after complete arterial injection of metacrylates with red dye, I proceeded with the dissection of the abdominal wall and observation, and extraction of the complete genital organs, fully injected.
If your objective is to remove only the ovaries, you might well gain practice and dissect directly from the lumbar dorsal wall, beneath the inferior pole of the kidneys.
I might find it easier to proceed with ventral laparotomy, to have a better view of the anatomical relations of the ovaries and oviduct.
Kind regards,
Maria
Here's some of the results I got, with the vascular injection techniques we currently use in the Dept. of Anatomy of the New University of Lisbon Medical School:
1. Ventral Abdominal Dissection after vascular injection: (I always keep most of the peritoneal fat, to avoid leaking of the injected dye)
2. Corrosion of all the organic tissues with strong acid or alcali (only the metacrylates resist, with the inner moulduing of the vascular casts.
Or Scanning electron Microscopic observatiion of corrosion casts,m after injection with Mercox:
Even if my work was mostly devoted to the vascular network of the uterus, you can find more images of these techniques, in the following link:
https://www.researchgate.net/publication/267568362_MvUt.EACA.2013
(Hope this was useful),
Kind regards,
Maria
Data MvUt.EACA.2013