27 October 2014 1 4K Report

We use the Langendorff mouse model.

Does anyone have a protocol for immunofluorescence staining of syndecan-1 in mouse hearts?

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Why does my IRC calculation generates higher energy structure around TS ?

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How to lyse nuclei without detergent?

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Does anyone know suppliers of high molecular weight PS?

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Homemade MDA-Assay protocol?

Dear all, Our group has tried for several months to establish a homemade protocol for MDA-assay (Malondialdehyde Colorimetric Assay) to measure lipid peroxidation levels in cell culture. So far,...

29 August 2023 7,697 3 View

Can somebody help with IDing this salp?

Dear all, I am curious to learn about the identification of the pictured salp individuum. The pictures were taken in October 2022 near San Antonio del Sur (southern Cuba) not far away from shore....

15 August 2023 9,008 0 View

Simulation Of Pump Down Graph with COMSOL 6.0?

hi community, I would like simulate the pump down graph of my vacuum pump. My first test is to use laminar flow, create an air cube of 1m^3 and add a vacuum pump on a side. The specificity of the...

13 June 2023 8,974 1 View

Which mEOS version to use for PALM?

Hi, I would like to use mEOS to tag proteins to evaluate their subcellular localization. I am new to PALM, and am a bit confused with all the different versions of mEOS available... mEOS2? mEOS4?...

19 April 2023 7,456 1 View

Question to all zooplankton and mollusc researchers: Can somebody help with IDing this small heteropod?

Dear colleagues, On an expedition in the southern Sargasso Sea, subtropical Northwest Atlantic, I encountered repeatedly specimens of the photographed heteropod, never (so far) being bigger than...

28 March 2023 3,057 4 View

Inconsistent results between plasmid dilutions?

Hello everyone, For the quantification of a product (mix of 2 plasmids) by qPCR/ddPCR, we obtained inconsistent results between the different 10 fold dilutions in nuclease-free water (dilution...

07 March 2023 6,798 2 View

STORM microscopy to image sfGFP or mVenus?

Hi, I was wondering if it is known if STORM microscopy could be used to obtain super-resolution images of sfGFP or mVenus tagged proteins in cells. I know it is more common to use fluorescent...

16 February 2023 982 2 View

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Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

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