I would like to know if it is possible to simulate how a protein folds during translation from the RNA sequence.
Also, being able to change parameters such as tRNA concentration based on codon frequencies, to regulate the rate of translation
The Sysmex DI-60 is a hematology analyzer developed and manufactured by Sysmex Corporation, a global leader in clinical laboratory solutions. The DI-60 is designed for use in medium-sized to large...
11 April 2024 1,666 1 View
Hello all! Finishing my research on black powder explosives. This spectrum is of black powder explosives and I can't seem to identify these peaks and could use some help.
16 February 2024 2,101 0 View
Hola! I'm having some issues interpreting 22.1 and 22.2 articles. From what I've read if the type species of a subdivision of a genus is NOT "listed" in the autonym subdivision, then that makes...
22 January 2024 3,247 6 View
What are the differences between a scoping, literature or mapping review?
21 November 2023 1,449 1 View
Hello everyone, I'm following a protocol in which 1 ml of diethyl ether is used as a triturating solvent. The product should remain a solid while the impurities should dissolve in the diethyl...
21 September 2023 8,919 4 View
Dear fellow researchers, I am currently writing a paper in cooperation with a company. For this, I want to compare a proceass of this "main" company with the same process of several other...
23 April 2023 596 2 View
I want to use the syncrosqueezed transform for analise 1-D and 2-D signal. I am wondering if you could recommend me some good texts on syncrosqueezed transform. Knowing if some code is available...
12 March 2023 5,692 3 View
I am working on design wavelet frames to detect specific patterns in 1-D signals. I wondering if you could recommend mesome good texts on wavelet frames construction. Knowing if some code is...
12 March 2023 3,278 1 View
Living bridges are a unique form of sustainable pedestrian infrastructure found in several parts of the world, including China, India, Indonesia, and Nepal. These bridges are created by manually...
11 March 2023 2,546 2 View
I want to use proteomics on mice sclera and western blotting to confirm the proteomics results . However, I am not sure how much mice sclera I need to get enough protein to be able to perform...
21 June 2022 9,575 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...
10 August 2024 4,347 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View