Jennifer Washburn

3 Questions 16 Answers 0 Followers

Questions related from Jennifer Washburn

Is there a non-toxic dye to visualize cannula placement in mouse brain?

I'm implanting Alzet pumps in mice with a brain infusion cannula to deliver into the ICV space. I am familiar with the technique that includes removing the pump from the tubing and then using a...

23 May 2024 7,165 3 View

How does the cell distinguish signals sent to different receptors, when many receptors converge on the same pathway?

There are 1000 7TM receptors, for example, but many of them exert their effects through G proteins which increase the cAMP in the cell. When cAMP increases, the cell reacts. But if two receptors...

14 October 2023 5,911 4 View

What is the cutoff between high affinity and low affinity protein interactions?

I know that if the affinity is too low, assays such as ELISA would not be appropriate, because the off rate of the ligand would be so fast that it would not hold on for the 2-3 hours that it takes...

28 August 2023 6,104 5 View