This is my first time culturing and working with bacteria.
I am culturing Bifidobacteria in the anaerobic gas chamber. According to the references I am using BL broth media supplemented with 5% defibrinated blood.
Previously while culturing other bacteria such as lactobacillus, I simply centrifuged the tube and add fresh media, dissolve the bacteria pellet and proceeded with further experiment.
In this case after 24-48hr incubation while centrifuging blood particles also collected at the bottom. Is it normal or I should follow some sp[ecial technique?
I am attaching the media details and references here for better understanding. Any suggestion is highly appreciated.
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The blood cells are going to be much much larger than the bacteria. You can removed them by a low speed centrifugation at a speed that won't pellet the bacteria (a few hundred RPM). Or by filtering through a large pore size filter that won't trap the bacteria. Then you can collect your bacteria by normal centrifugation and proceed as usual.
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