Hi, I did 16s rRNA sequencing and my isolate showed b.breve strain 3018. After doing the nacl resistance test it turned out that my strain is resistant at concentrations of 1.5%, 2.5%, up to 3.5%. My friend and I have bifidobacterium breve isolate with different strains but our nacl resistance ability is different, my friend's isolate is not resistant to nacl. my question is there a difference in the gene/genome or what? If yes, what is the name of the gene/genome? because I find it difficult to find specific literacy mentioning the genes/genomes that play a role in nacl resistance
note: I did a nacl test for probiotic characterization
Thank You
Bifidobacterium breve is a type of beneficial bacteria commonly found in the human gut. It exhibits certain characteristics that contribute to its resistance to NaCl (salt) concentrations up to 3.5%. Here are some factors that may contribute to Bifidobacterium breve's resistance:
1. Osmotic Adaptation: Bifidobacterium breve has mechanisms to adapt and survive in high-salt environments. It can adjust its internal osmolarity to balance the external salt concentrations, allowing it to maintain cellular integrity and function.
2. Salt Tolerance Mechanisms: Bifidobacterium breve possesses specific mechanisms that protect its cellular structures from salt-induced damage. This includes the synthesis and accumulation of compatible solutes (osmoprotectants), such as glycine betaine or trehalose, which help maintain cellular hydration and stability in high-salt conditions.
3. Membrane Stability: The cell membrane of Bifidobacterium breve contains specific lipid compositions and protein structures that contribute to its stability and resistance to salt stress. These adaptations prevent the leakage of intracellular components and maintain the integrity of the cell.
4. Enzymatic Defense Systems: Bifidobacterium breve may possess enzymatic systems that help neutralize or counteract the harmful effects of salt. These enzymes can contribute to the protection of cellular macromolecules and maintain cellular homeostasis.
It's important to note that the specific mechanisms of salt resistance in Bifidobacterium breve may vary and are still being studied. The resistance to high NaCl concentrations is likely a result of the interplay between multiple factors that allow the bacterium to thrive in salt-rich environments.
It is worth mentioning that the ability of Bifidobacterium breve to tolerate high salt concentrations is beneficial in certain applications, such as the production of fermented foods and probiotic formulations, where salt is commonly used as a preservative or flavoring agent.
Los microorganismos no halófilos capaces de crecer tanto en ausencia como en presencia de sal son llamados halotolerantes; los halotolerantes que son capaces de crecer aproximadamente al 15% (w/v) de NaCl (2,5 M) son considerados halotolerantes extremos. Los microorganismos que requieren sal para su crecimiento son llamados halófilos. De acuerdo a la definición de Kushner (30), es posible distinguir entre halófilos débiles, como lo son la mayoría de los organismos marinos, considerando que el agua de mar contiene cerca del 3% (w/v) de NaCl; halófilos moderados, cuyo crecimiento óptimo se encuentra en un rango del 3 al 15% (w/v) de sal, y halófilos extremos, que presentan un crecimiento óptimo al 25% (w/v) de NaCl (44).
Por otro lado debes aislar el ADN de ambas cepas y amplificar genes de resistencia a salinidad, a través de PCR convencional o Real Time. Estos genes ya determinados por otros autores cuyas secuencia a veces la publican.
Non-halophilic microorganisms capable of growing both in the absence and in the presence of salt are called halotolerant; halotolerants that are capable of growing in approximately 15% (w/v) NaCl (2.5 M) are considered extreme halotolerants. Microorganisms that require salt for their growth are called halophiles. According to Kushner's definition (30), it is possible to distinguish between weak halophiles, as most marine organisms are, considering that seawater contains about 3% (w/v) of NaCl; moderate halophiles, whose optimum growth is found in a range of 3 to 15% (w/v) of salt, and extreme halophiles, which show optimal growth at 25% (w/v) of NaCl (44).
On the other hand, you must isolate the DNA of both strains and amplify genes for resistance to salinity, through conventional or Real Time PCR. These genes already determined by other authors whose sequence is sometimes published.
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