11 October 2019 1 10K Report

Can anyone suggest an antibody for Piezo1 that has been used in good publications for immunoflourescence stainings?

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What is accurate methode to set up two electrode device in supercapcitor device?

I made two electrode devices for the supercapacitor. I combined activated carbon and PVDF for negative electrodes in an 80:10:10 ratio. I combined active working material, PVDF, and activated...

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How to import file on xpspeak41?

I want to analyse XPS spectra from xpspeak41. I try every file format such as ASC, .dat but it always shows an error reading file. what should I do? please see the images I attached below

11 February 2024 6,860 3 View

How can I make different concentrations of antigen solution from a stock?

I have 50 ug of Human Her2 / ErbB2 Protein, Tag Free antigen in white powder form and need to make various concentrations of that antigen, such as 50 unit/ml, 10 unit/ml, 0.01 unit/ml, and 0.1...

05 February 2024 9,048 6 View

How can we calculate the amount of shelling precursor for the synthesis of core-shell nanoparticles to get particular shelling thickness?

We have the diameter of the core nanoparticles.

19 November 2023 3,113 3 View

Why does the Screen printed carbon electrode (SPCE) Dropsen 110 fail to achieve the expected results on the Metrohm potentiostat?

For my experiment, I'm currently using a Dropsen 110 Screen-printed carbon electrode (SPCE). I need to take my material's CV, DPV, and EIS. But I can't seem to find the right DPV. The current...

08 October 2023 4,435 8 View

Why is Mxene morphology always in nanosheets?

Why is MXene morphology always in nanosheets? Can it be spherical, Core-shell, cubic or needle-shaped?

03 September 2023 7,197 9 View

What is the molecular identity and function of Endothelial Barrier antigen (EBA)?

First reported in 1987, the Endothelial Barrier Antigen (EBA) is expressed in brain endothelium and is downregulated in ischemic stroke. Its expression is negatively correlated with BBB...

30 August 2023 5,648 1 View

Is it possible to assess BBB permeability in mice using intraperitoneal injection of tracers?

Fluorescent tracers (sodium fluorescein and Dextrans) are usually injected into the lateral tail vein of the mouse. The tracers are allowed to circulate for 30 minutes before trans cardiac...

13 August 2023 7,782 0 View

Can I perform immunofluroscent staining in 96 well plate?

I am planning to stain a cell surface protein by immunofluorescence and then take readouts by plate reader. As I would like to detect cell surface protein, I guess cells should not be fixed. I...

07 August 2023 9,681 5 View

How do you convert fg/ml, ng/ml, and microgram/ml to IU/ml?

I need to convert the fg/ml, and ng/ml limit of detection into IU/ml. What is the formula for converting fg/ml, ng/ml limit of detection into IU/ml?

06 August 2023 9,002 4 View

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How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

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Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

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Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to make a Lateral flow control line more uniform?

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The use of data from PubChem for commercial purposes?

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Suggestions for the Annexin V Positive Control ?

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