11 October 2019 1 10K Report

Can anyone suggest an antibody for Piezo1 that has been used in good publications for immunoflourescence stainings?

More Divija Deshpande's questions See All
Is there any website or online source to draw the architecture of the neural network ?

I have the details of the input, output, number of hidden nodes and number of hidden layers data. How can I draw the architecture of the neural network for my condition.

21 October 2020 6,648 1 View

How to model geosynthetics in stone column encasement (abaqus)?

I am using abaqus to model geosynthetic encased stone column (wished in-place). I am new to abaqus so how should I model geosynthetics in 3D and 2D (solid or shell or wire ) and can I get an idea...

17 September 2020 5,165 3 View

Is it possible to get the probable lattice structure of crystalline powder sample using miller indices (h, k, l values) and XRD data?

Hello everyone, presently, I am working on crystalline properties of some polymers with respect to their individual treatments using X-Ray Diffraction (XRD). Is there any software which can give...

08 September 2020 1,223 5 View

How to increase the yield of extracellular DNA in extraction process from water samples?

I am using 1% CTAB for extracellular DNA extraction, followed by isopropanol precipitation, phenol chloroform extraction, ethanol precipitation and wash. How can I increase my yield?

02 March 2020 7,947 3 View

How to fix the Open cv module error?

I am working on image segmentation using python open cv package but couldn't able to work with it because of error indicating "ModuleNotFoundError: No module named 'cv2'" and the same error is...

02 February 2020 928 4 View

How can I use sonication to get lysosomal membrane protein?

I am trying to standardise lysosomal enzymes assay. Since the enzyme is active inside the lysosome and is partially embedded in the lysosomal membrane, lysis of cells with lysosomal membrane is...

10 February 2019 7,653 2 View

I have completed my bachelor's in Electrical Engineering and I am looking forward to start learning more about smart grid .From where should I start.?

I want to learn about smart grid and simulating a smart grid network and also the data analytics used in smart grid network .

23 May 2018 6,165 3 View

Is fluorescence from boiled residues of sugars/oils/acids due to carbon quantum dots or some other fluorescent compounds?

I tried making carbon quantum dots by microwaving aqueous solutions of citric acid as a simple science outreach activity. I followed this literature precedent ( Milosavljevic, V., et al. J...

23 May 2018 950 3 View

Does sequential treatment for monocytes differentiation in to macrophages (in vitro) affect the generation of cytokine pattern after stimulation?

I would like to know if in vitro derived macrophages (from monocytic cell line) by the treatment of 2 stimulants/chemicals (for differentiation) subsequently would affect the cytokine profile...

22 March 2018 5,029 2 View

What is the ideal computer configuration for carrying out numerical relativity simulations of astrophysical objects with python and cactus framework?

I am interested to know computing architectures required to solve Einstein's equations in General Relativity with applications to black hole and gravitational waves

07 January 2018 1,912 1 View

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Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

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