Divija Deshpande

3 Questions 4 Answers 0 Followers

Questions related from Divija Deshpande

Is there a good Piezo1 antibody available commercially?

Can anyone suggest an antibody for Piezo1 that has been used in good publications for immunoflourescence stainings?

11 October 2019 10,149 1 View

How can we delete genes in primary DRG cultures?

What is the best possible method to knockout genes fro a primary DRG neuronal culture? ..Apart from extracting DRGs from a knockout mouse..Since a knockout mouse is unavailable for the gene that I...

10 September 2019 1,387 4 View

What would be be an optimal method to extract peptides from ms DRG, nerves and footpads, later to be used in ELISA ?

RIPA extraction works very well for western blot, but not with ELISA. I have read about acetic acid or TFA extraction protocols, however, they are not suitable to maintain protein stability. I am...

13 September 2016 9,351 2 View